Micro spin cup
| #400 050 JETQUICK Plasmid Miniprep Spin Kit / 50 110 USD |
| #400 250 JETQUICK Plasmid Miniprep Spin Kit / 250 328 USD |
| #420 050 JETQUICK Gel Extraction Spin Kit / 50 117 USD |
| #420 250 JETQUICK Gel Extraction Spin Kit / 250 350 USD |
| #410 050 JETQUICK PCR Purification Spin Kit / 50 117 USD |
| #410 250 JETQUICK PCR Purification Spin Kit / 250 350 USD |
| Specifications: | |
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Product is designed to prepare plasmid DNA (small scale) from E.coli cells |
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Product contents are micro spin cups, RNAse A, ready-to-use buffers and protocol |
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Very high DNA capacity (up to 50 µg plasmid DNA per micro-spin cup) |
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Ultra quick procedure using micro spin cups |
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No chromosomal DNA contamination |
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No ethanol or isopropanol precipitation |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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Excellent reproducibility |
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Culture volumes up to 5ml |
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Total procedure is < 25min |
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Plasmid sizes are up to 20.000bp |
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Plasmid recovery is 90% - 95% on average |
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Plasmid quality matches CsCl purified DNA |
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A260/280 ratio is > 1.8 |
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DNA elution in TE buffer or equivalent low-salt buffer or simply water |
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Micro-spin cups are adaptable to vacuum manifold (see protocol links below) |
| Applications: | |
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Fluorescent sequencing |
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Ligation |
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Restriction analysis |
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PCR template preparation |
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Labeling |
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All other enzymatic reactions |
A number of twelve plasmid preparations were carried out simultaneously from twelve separate 1 ml E.coli cultures using the JETQUICK Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose ge