LFU-Unit Midi column Maxi column
| #211 025 JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25 256 USD |
| #211 100 JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100 761 USD |
| #221 020 JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20 401 USD |
| #221 100 JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 100 1484 USD |
| Advantages: | |
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Very convenient procedure - much simpler and shorter than any competitor's protocol |
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Great time-saving compared to standard procedure |
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Newly designed FAST Midi columns much faster than any comparable product |
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Larger culture volumes can be processed on the new FAST Midi columns |
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No centrifugation necessary to clear bacterial lysate |
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Lysate clarification integrated into column procedure |
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Only ONE column wash for the new FAST Midi and Maxi columns |
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No clogging of the lysate filter unit |
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Much less susceptible against overloading than competitor's products |
| JETSTAR specifications: | |
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Product is designed to prepare plasmid DNA (medium and large scale) from E.colicells |
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Product contents are gravity fast flow columns, RNase A, ready-to-use buffers and protocol |
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JETSTAR / LFU preparations are available in Midi and Maxi format |
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JETSTAR is able to prepare cosmid DNA and BAC DNA as well |
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No chromosomal DNA contamination |
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No phenol/chloroform extraction |
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No organic or toxic reagents |
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Excellent reproducibility |
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Total procedure in 30 - 40 min |
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Plasmid DNA is ultrapure (better than 2 x CsCl) |
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Plasmid DNA recovery is 90% - 95% on average |
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Plasmid DNA of all sizes can be prepared |
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Plasmid DNA yields are up to 200 µg (Midi) and up to 850 µg (Maxi) |
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A260/280 ratio is > 1.85 |
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Endotoxin level is < 0.01 EU/µg plasmid DNA |
| Applications for plasmid and cosmid DNA | |
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Transfection |
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Manual and automated sequencing, including capillary sequencing |
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Microinjection |
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In vitro transcription |
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Restriction analysis, ligation, labeling etc. |
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Transfection of endotoxin-sensitive cells (Endo Free) |
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Gene therapy applications |