Acryl-AquaClean

Microbiocidal additive special for fluids in baths made of acrylic and aluminium.
 
 
AAC-mali
 
 
 
Cat # Quantity                                   Price
WAK-AQA-250-50L 250 ml concentrate                189 USD
 
 
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AquaClean

AquaClean
   

 

Microbiocidal additive for heating baths made of stainless steel. Inhibits growth of bacteria, viruses, algae, fungi and precipitation of inorganic salt

AC-mali

 

Cat # Quantity                                                                                       Price
WAK-AQ-250-50L 250 ml concentrate                                                               126 USD

 

 

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AquaClean

AquaClean
   

 

Microbiocidal additive for heating baths made of stainless steel. Inhibits growth of bacteria, viruses, algae, fungi and precipitation of inorganic salt

AC-mali

 

Cat # Quantity                                                                                       Price
WAK-AQ-250-50L 250 ml concentrate                                                               126 USD

 

 

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Biocidal ZF

 
Biocidal ZF
 BC-mali   
 Cat  #                   Quantity                 Price
 
WAK-ZF-1               1 x 1 lit.                   156 USD 
WAK-ZF-12           12 x 0,2 lit.                338 USD
WAK-ZF-6               6 x 1 lit.                  422 USD
 
Special spray-disinfectant incubators and laminar flow hoods for protecting cultures against bacteria, fungi and  enveloped viruses.
 
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MEDIA IN POWDERED FORM

DMEM HIGH GLUCOSE (1 g/l)  
       
  SIZE CATALOGUE
NUMBER		 EURO
DMEM powder 10 lit G31-802 45$
with L-Glutamine 50 lit G35-802 70$
       
       
       
DMEM HIGH GLUCOSE (4,5 g/l)  
       
  SIZE CATALOGUE
NUMBER		 EURO
DMEM powder 10 lit G31-803 45$
with L-Glutamine 50 lit G35-803 70$
DMEM powder 10 lit G31-804 47$
with Sodium Pyruvate
with L-Glutamine		 50 lit G35-804 82$
       
       
DMEM / HAM’S F-12 MIX (1:1)  
       
  SIZE CATALOGUE
NUMBER		 EURO
DMEM/Ham’s F-12 powder 10 lit G31-006 45$
without L-Glutamine 50 lit G35-006 70$
       
HAM’S F-10 AND HAM’S F-12    
       
  SIZE CATALOGUE
NUMBER		 EURO
Ham's F-10 powder 10 lit G31-807 45$
with L-Glutamine 50 lit G35-807 70$
Ham's F-12 powder 10 lit G31-808 45$
with L-Glutamine 50 lit G35-808 70$
       
ISCOVE’S MODIFIED DMEM (IMDM)  
       
  SIZE CATALOGUE
NUMBER		 EURO
IMDM powder 10 lit G31-809 55$
without supplements
with L-Glutamine		 50 lit G35-809 135$
       
       
       
MEM WITH EARLE’S SALTS    
       
  SIZE CATALOGUE
NUMBER		 EURO
MEM powder 10 lit G31-812 45$
with Earle's Salts
with L-Glutamine		 50 lit G35-812 70$
       
       
MEDIUM 199 WITH EARLE’S SALTS  
       
  SIZE CATALOGUE
NUMBER		 EURO
Medium 199 powder 10 lit G31-816 45$
with Earle's Salts
with L-Glutamine		 50 lit G31-816 70$
       
       
RPMI 1640      
       
  SIZE CATALOGUE
NUMBER		 EURO
RPMI 1640 powder 10 lit G31-819 45$
with L-Glutamine 50 lit G35-819 70$
RPMI 1640 powder 10 lit G31-020 55$
with HEPES, with L- Glutamine 50 lit G35-020 125$
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Buffer solutions

BASAL SALTS SOLUTIONS  
       
HANKS’ BUFFERED SALT SOLUTION (HBSS)  
       
  SIZE CATALOGUE
NUMBER		 EURO
Hanks` BSS (1x)
with Ca & Mg
with Phenol Red		 10x500 ml H15-007 125$
Hanks` BSS (1x)
with Ca & Mg
without Phenol Red		 10x500 ml H15-008 120$
10x1000 ml H21-008 140$
Hanks` BSS (1x)
without Ca & Mg
without Phenol Red		 10x500 ml H15-009 120$
10x1000 ml H21-009 140$
Hanks` BSS (1x)
without Ca & Mg
with Phenol Red		 10x500 ml H15-010 120$
Hanks` BSS (10x)
without Ca & Mg
without Phenol Red		 10x500 ml H15-012 185$
10x1000 ml H21-012 265$
       
       
DULBECCO’S PBS      
       
  SIZE CATALOGUE
NUMBER		 EURO
Dulbecco's PBS (1x)
with Ca/Mg		 10x500 ml H15-001 120$
10x1000 ml H21-001 140$
Dulbecco's PBS (1x)
without Ca & Mg		 10x500 ml H15-002 120$
10x1000 ml H21-002 140$
Dulbecco's PBS (10x)
without Ca & Mg		 10x500 ml H15-011 185$
10x1000 ml H21-011 265$
       
       
SOLUTIONS IN CONTAINERS    
       
  SIZE CATALOGUE
NUMBER		 EURO
Dulbecco's PBS (1x)
without Ca & Mg		 10 L H31-002 85$
Dulbecco's PBS (1x)
without Ca & Mg		 20 L H32-002 100$
       
       
STERILE WATER IN CONTAINERS  
       
  SIZE CATALOGUE
NUMBER		 EURO
Sterile Water
EP Grade		 10x500 ml S15-012 120$
Sterile Water
EP Grade		 10x1000 ml S21-012 145$
Sterile Water
EP Grade		 10 L S31-012 83$
Sterile Water
EP Grade		 20 L S32-012 98$
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SERA

DEFINED FOETAL BOVINE SERUM  
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum "GOLD" 10x100 ml A11-649 180$
Category "EU" 10x500 ml A15-649 465$
Foetal Bovine Serum "GOLD" 10x100 ml A11-609 405$
USA origin 10x500 ml A15-609 1325$
Foetal Bovine Serum "GOLD" 10x100 ml A11-629 on request
AUSTRALIA origin 10x500 ml A15-629
       
       
FOETAL BOVINE SERUM     
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum  10x100 ml A11-043 170$
Category "EU" 10x500 ml A15-043 420$
Foetal Bovine Serum  10x100 ml A11-003 385$
USA origin 10x500 ml A15-003 1295$
Foetal Bovine Serum  10x100 ml A11-023 on request
AUSTRALIA origin 10x500 ml A15-023
       
       
GAMMA IRRADIATED    
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum γ-irradiated 100 ml A11-346 100$
Category "EU" 500 ml A15-346 145$
Foetal Bovine Serum γ-irradiated 100 ml A11-306 125$
USA origin 500 ml A15-306 232$
Foetal Bovine Serum γ-irradiated 100 ml A11-326 on request
AUSTRALIA origin 500 ml A15-326
       
       
HEAT INACTIVATED    
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum 
Heat Inactivated		 10x100 ml A11-144 185$
Category "EU" 10x500 ml A15-144 485$
Foetal Bovine Serum 
Heat Inactivated		 10x100 ml A11-104 405$
USA origin 10x500 ml A15-104 1355$
Foetal Bovine Serum 
Heat Inactivated		 10x100 ml A11-124 on request
AUSTRALIA origin 10x500 ml A15-124
Foetal Bovine Serum Gold
Heat Inactivated		 10x100 ml A11-664 195$
Category "EU" 10x500 ml A15-664 525$
Foetal Bovine Serum Gold
Heat Inactivated		 100 ml A11-614 415$
USA origin 500 ml A15-614 1395$
Foetal Bovine Serum Gold
Heat Inactivated		 10x100 ml A11-634 on request
AUSTRALIA origin 10x500 ml A15-634
Foetal Bovine Serum Mycoplex
Heat Inactivated		 10x100 ml A11-774 197$
Category "EU" 10x500 ml A15-774 545$
Foetal Bovine Serum Mycoplex
Heat Inactivated		 100 ml A11-784 120$
USA origin 500 ml A15-784 220$
Foetal Bovine Serum Mycoplex
Heat Inactivated		 100 ml A11-794 on request
AUSTRALIA origin 500 ml A15-794
       
       
DIALYSED      
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum 
Dialysed		 10x100 ml A11-245 295$
Category "EU" 10x500 ml A15-245 895$
Foetal Bovine Serum 
Dialysed		 10x100 ml A11-205 505$
USA origin 10x500 ml A15-205 1755$
Foetal Bovine Serum 
Dialysed		 10x100 ml A11-225 on request
AUSTRALIA origin 10x500 ml A15-225
       
       
MYCOPLEX™      
       
  SIZE CATALOGUE
NUMBER		 EURO
Mycoplex™ Foetal Bovine Serum 10x100 ml A11-773 205$
Category “EU” 10x500 ml A15-773 535$
Mycoplex™ Foetal Bovine Serum 10x100 ml A11-771 415$
USA origin 10x500 ml A15-771 1405$
Mycoplex™ Foetal Bovine Serum 10x100 ml A11-772 on request
AUSTRALIA origin 10x500 ml A15-772
       
       
PRETESTED      
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum 100 ml A11-080 65$
pretested on 
Embryonic Stem Cells 		 500 ml A15-080 195$
Foetal Bovine Serum 10x100 ml A11-082 195$
pretested on Amnion Cells  10x500 ml A15-082 535$
Foetal Bovine Serum 100 ml A11-050 115$
Tetracycline negative 500 ml A15-050 155$
       
       
IgG STRIPPED      
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum
IgG Stripped		 10x100 ml A11-548 395$
Category: EU 10x500 ml A15-548 1145$
Foetal Bovine Serum
IgG Stripped		 10x100 ml A11-508 575$
USA origin 10x500 ml A15-508 2175$
Foetal Bovine Serum
IgG Stripped		 10x100 ml A11-528 on request
AUSTRALIA origin 10x500 ml A15-528
       
       
EMEA TESTED      
       
  SIZE CATALOGUE
NUMBER		 EURO
Foetal Bovine Serum 10x100 ml A11-032 on request
EMEA tested
AUSTRALIA origin		 10x500 ml A15-032
Foetal Bovine Serum
EMEA tested
AUSTRALIA origin		 10x100 ml A11-331 on request
Gamma irradiated 10x500 ml A15-331
       
       
BOVINE SERUM      
       
  SIZE CATALOGUE
NUMBER		 EURO
Newborn Calf Serum 10x100 ml B11-001 125$
  10x500 ml B15-001 205$
Newborn Calf Serum 10x100 ml B11-102 135$
Heat Inactivated 10x500 ml B15-102 245$
Newborn Calf Serum 10x100 ml B11-303 180$
Gamma Irradiated 10x500 ml B15-303 435$
       
       
  SIZE CATALOGUE
NUMBER		 EURO
Calf Serum 10x100 ml B11-004 130$
  10x500 ml B15-004 235$
Calf Serum 10x100 ml B11-106 135$
Heat Inactivated 10x500 ml B15-106 255$
Calf Serum 10x100 ml B11-307 200$
Gamma Irradiated 10x500 ml B15-307 535$
       
       
  SIZE CATALOGUE
NUMBER		 EURO
Donor Calf Serum 10x100 ml B11-008 145$
  10x500 ml B15-008 315$
Donor Calf Serum 10x100 ml B11-109 155$
Heat Inactivated 10x500 ml B15-109 355$
Donor Calf Serum 10x100 ml B11-310 215$
Gamma Irradiated 10x500 ml B15-310 585$
       
       
BOVINE SERUM      
       
  SIZE CATALOGUE
NUMBER		 EURO
Bovine Serum 10x100 ml B11-011 130$
  10x500 ml B15-011 235$
Bovine Serum 10x100 ml B11-112 135$
Heat Inactivated 10x500 ml B15-112 255$
Bovine Serum 10x100 ml B11-313 185$
Gamma Irradiated 10x500 ml B15-313 485$
       
       
HORSE SERUM      
       
  SIZE CATALOGUE
NUMBER		 EURO
Horse Serum 10x100 ml B11-021 135$
  10x500 ml B15-021 245$
Horse Serum 10x100 ml B11-122 145$
Heat Inactivated 10x500 ml B15-122 265$
Donor Horse Serum 10x100 ml B11-023 165$
  10x500 ml B15-023 385$
Donor Horse Serum 10x100 ml B11-124 175$
Heat Inactivated 10x500 ml B15-124 415$
       
       
OTHER ANIMAL SERA    
       
  SIZE CATALOGUE
NUMBER		 EURO
Porcine Serum 10x100 ml B11-030 155$
  10x500 ml B15-030 320$
Porcine Serum 10x100 ml B11-331 220$
Gamma Irraditated 10x500 ml B15-331 595$
Lamb Serum 10x100 ml B11-032 235$
  10x500 ml B15-032 655$
Chicken Serum 10x100 ml B11-033 255$
  10x500 ml B15-033 740$
Rabbit Serum 10x100 ml B11-034 200$
Goat Serum 10x100 ml B11-035 255$
  10x500 ml B15-035 725$
Guinea Pig 10x20 ml B02-036 295$
  10x50 ml B05-036 620$
Rat Serum 10x20 ml B02-037 585$
Mouse Serum 10x20 ml B02-038 445$
Dog Serum 10x10 ml B01-040 235$
  10x50 ml B05-040 655$
       
Other animal sera are available upon special request.  
       
       
HUMAN  SERUM       
       
  SIZE CATALOGUE
NUMBER		 EURO
Human Serum 100 ml C11-020 150$
“off the clot” 500 ml C15-020 315$
Human Serum "off the clot", 100 ml C11-021 175$
Type AB 500 ml C15-021 410$
Human Serum “converted” 100 ml C11-001 130$
  500 ml C15-001 235$
Human Serum, Type AB, 100 ml C11-002 135$
"converted" 500 ml C15-002 250$
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Reagents

ANTIBIOTICS AND ANTIMYCOTICS  
       
  SIZE CATALOGUE
NUMBER		 EURO
Amphotericin B 10x100 ml P11-001 185$
Antibiotic/Antimycotic Solution 10x100 ml P11-002 175$
Gentamicin (10 mg/ml) 10x100 ml P11-004 485$
Gentamicin (50 mg/ml) 100 ml P11-005 285$
Penicillin/Streptomycin (100x) 10x100 ml P11-010 125$
L-Glutamine (200mM) with
Penicillin/Streptomycin (100x)  		 10x100 ml P11-013 165$
       
       
DETACHMENT ENZYMES    
       
TRYPSIN / TRYPSIN EDTA    
       
  SIZE CATALOGUE
NUMBER		 EURO
Trypsin (1:250) (10x) 
2,5% in DPBS		 10x100 ml L11-001 205$
Trypsin (1:250) (1x)
0,25% in DPBS		 10x100 ml L11-002 120$
Trypsin EDTA (10x)
0,5% / 0,2% in DPBS		 10x100 ml L11-003 205$
Trypsin EDTA (1x)
0,05% / 0,02% in DPBS		 10x100 ml L11-004 120$
       
       
TRYPSIN UV-INACTIVATED    
       
  SIZE CATALOGUE
NUMBER		 EURO
Trypsin (1:250)
UV-inactivated (10x) 		 10x100 ml L11-657 255$
Trypsin (1:250)
UV-inactivated (1x) 		 10x100 ml L11-658 135$
Trypsin EDTA (1:250)
UV-inactivated (10x) 		 10x100 ml L11-659 255$
Trypsin EDTA (1:250)
UV-inactivated (1x) 		 10x100 ml L11-660 135$
       
       
COLLAGENASE      
       
  SIZE CATALOGUE
NUMBER		 EURO
Collagenase Type 2 1 g K01-040-2 270$
20 ml K05-039-2 215$
       
       
ACCUTASE - ACCUMAX    
       
  SIZE CATALOGUE
NUMBER		 EURO
Accutase-Solution 10x100 ml L11-007 265$
Accutase 2-Solution 10x100 ml L11-012 185$
AccuMax-Solution 10x20 ml L02-008 315$
AccuMax-Solution 100 ml L11-008 185$
       
       
AMINO ACID SOLUTIONS    
       
  SIZE CATALOGUE
NUMBER		 EURO
MEM Amino Acids (50x) 10x100 ml M11-002 135$
MEM Non essential
Amino Acids (100x)		 10x100 ml M11-003 90$
L-Glutamine 200 mM (100x) 10x100 ml M11-004 90$
       
       
SEPARATION MEDIA      
       
  SIZE CATALOGUE
NUMBER		 EURO
LSM 1077
Lymphocyte Separation Medium		 10x100 ml J11-004 165$
LSM 1077
Lymphocyte Separation Medium		 10x500 ml J15-004 375$
       
       
BOVINE SERUM ALBUMIN  
       
  SIZE CATALOGUE
NUMBER		 EURO
Bovine Serum Albumin 100 g K41-001-100 144$
pH 7.0 500 g K41-001-500 295$
Bovine Serum Albumin 100 g K41-002-100 160$
Fatty Acid free 500 g K41-002-500 395$
Bovine Serum Albumin 10x100 ml K11-013 230$
30% Solution, sterile 10x50 ml K05-013 170$
Bovine Serum Albumin 100 g K41-011-100 155$
low Endotoxin content 50 g K41-011-50 125$
       
       
ADDITIVES  
       
  SIZE CATALOGUE
NUMBER		 EURO
MEM Vitamins (100x) 10x100 ml N11-002 125$
HEPES Buffer Solution
(1M=238.5 mg/ml)		 10x100 ml S11-001 295$
Sodium Pyruvate Solution
(100mM=11mg/ml)		 10x100 ml S11-003 125$
Hybridoma Cloning Factor 10 ml S01-015 130$
  50 ml S05-015 255$
Potassium Chloride 10x100 ml S11-018 115$
Giemsa Staining Solution 10x100 ml  S11-019 185$
Colcemid 10x10 ml J01-003 125$
Phytohaemagglutinin 10x10 ml J01-006 255$
       
       
SERUM SUBSTITUTE      
       
  SIZE CATALOGUE
NUMBER		 EURO
SerEx! 10x100 ml F11-001 235$
Serum Substitute 10x500 ml F15-001 685$
Lipumin 100 ml F11-011 135$
Growth Additive 500 ml F15-011 275$
       
       
TRANSFECTION      
       
  SIZE CATALOGUE
NUMBER		 EURO
G-418-Sulphate 1 g P21-011 85$
5 g P25-011 175$
10 g P27-011 275$
G-418-Sulphate 
Solution (50mg/ml)		 20 ml P02-012 125$
100 ml P11-012 275$
Hygromycin B 500 mg P05-014 85$
1g P21-014 110$
Hygromycin B
Solution (50mg/ml)		 20ml P02-015 165$
Nanofectin 0,5 ml Q005-001 165$
1 ml Q01-001 230$
MycoKill AB 100 ml P11-016 110$
Blasticidin 50 mg P05-017 184$
100 mg P11-017 265$
Puromycin 100 mg P11-019 155$
500 mg P15-019 375$
Carbenicillin 1 g P21-020 123$
5 g P25-020 230$
10 g P31-020 350$
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Normal endometrium

Category Human tissue array
   Cat. No. CYN
   Product description Normal endometrium
   Data sheet CYN1
CYN1
  general_information
   No. of samples 59
   Text
Current version: CYN1 
Normal endometrium: 59 cores

price : 5 slide for 600 $

CYN1  Human, Normal Endometrium
         
No. Age Sex Phase Associated lesion
1 35 F proliferative  squamous cell carcinoma of cervix
2 39 F proliferative  leiomyoma
3 40 F proliferative  squamous cell carcinoma in situ of cervix
4 40 F secretory leiomyoma
5 33 F secretory leiomyoma
6 45 F secretory leiomyoma
7 40 F secretory leiomyoma
8 39 F secretory leiomyoma
9 38 F proliferative  endometriosis, ovary
10 42 F secretory leiomyoma
11 44 F secretory leiomyoma
12 45 F secretory leiomyoma
13 37 F secretory leiomyoma
14 38 F proliferative  leiomyoma
15 38 F proliferative  leiomyoma
16 42 F secretory squamous cell carcinoma of cervix
17 38 F proliferative  leiomyoma
18 37 F proliferative  leiomyoma
19 41 F proliferative  leiomyoma
20 34 F secretory squamous cell carcinoma in situ of cervix
21 43 F secretory leiomyoma
22 40 F proliferative  leiomyoma
23 42 F secretory leiomyoma
24 41 F proliferative  moderate dysplasia of cervix
25 45 F proliferative  leiomyoma
26 30 F secretory squamous cell carcinoma of cervix
27 39 F proliferative  leiomyoma
28 43 F secretory leiomyoma
29 36 F proliferative  leiomyoma
30 44 F secretory leiomyoma
31 42 F proliferative  leiomyoma
32 30 F secretory leiomyoma
33 41 F secretory leiomyoma
34 33 F secretory leiomyoma
35 37 F secretory normal
36 29 F secretory squamous cell carcinoma in situ of cervix
37 42 F secretory leiomyoma
38 37 F proliferative  squamous cell carcinoma in situ of cervix
39 44 F proliferative  leiomyoma
40 35 F proliferative  adenomyoma
41 32 F secretory leiomyoma
42 36 F secretory cellular leiomyoma
43 37 F secretory leiomyoma
44 35 F secretory leiomyoma
45 37 F secretory squamous cell carcinoma in situ of cervix
46 42 F secretory leiomyoma
47 44 F proliferative  leiomyoma
48 40 F proliferative  cellular leiomyoma
49 35 F proliferative  squamous cell carcinoma of cervix
50 36 F proliferative  squamous cell carcinoma of cervix
51 35 F secretory squamous cell carcinoma of cervix
52 43 F secretory squamous cell carcinoma of cervix
53 42 F proliferative  leiomyoma
54 35 F proliferative  squamous cell carcinoma of cervix
55 43 F secretory leiomyoma
56 42 F secretory squamous cell carcinoma of cervix
57 33 F proliferative  leiomyoma
58 35 F proliferative  adenomyosis
59 45 F secretory leiomyoma
60     carbon  

General Information of the Tissue Array Slide :

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
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Skin cancer

Category Human tissue array
   Cat. No. CX
   Product description Skin cancer
   Data sheet CX1
CX1
   
  general_information
   No. of samples 59
   Text
Current version: CX1 
Skin cancer: 50 cores 
Normal skin: 9 cores

Price : 5 slide for 600 $

CX1  Human, skin cancer
           
  Age Sex Diagnosis Site Stage
1 53 M squamous cell carcinoma, well differentiated skin, face T2N0M0
2 54 M squamous cell carcinoma, well differentiated skin, scalp T3N0M0
3 62 M squamous cell carcinoma, well differentiated skin, neck T2N0M0
4 58 F squamous cell carcinoma, well differentiated skin, foot TXNXMX
5 55 M squamous cell carcinoma, moderately differentiated skin neck T4N0M0
6 60 M squamous cell carcinoma, moderately differentiated skin, face T2N0M0
*7 57 M squamous cell carcinoma, moderately differentiated pharynx T4N0M0
8 61 M squamous cell carcinoma, well differentiated skin elbow T4N1M0
*9 25 F metastatic squamous cell carcinoma skin, scalp  
10 67 M squamous cell carcinoma, well differentiated skin, neck T4N0M0
11 63 M squamous cell carcinoma, poorly differentiated skin, perianal T2NXM0
12 63 M recurrent squamous cell carcinoma skin, face T4NXM0
*13 87 F squamous cell carcinoma, well differentiated skin face T1N0M0
*14 66 M squamous cell carcinoma, poorly differentiated skin, neck T4NXM0
15 48 M squamous cell carcinoma skin, axilla T4N12M1
16 65 M squamous cell carcinoma anus TXNXMX
*17 67 M metastatic squamous cell carcinoma skin, chest wall 3.5 cm
18 48 M squamous cell carcinoma, moderately differentiated skin, lower leg T3N0M0
19 51 F squamous cell carcinoma, well differentiated skin, thigh T3N0M0
20 40 M squamous cell carcinoma, well differentiated skin, knee T4N0M0
21 40 M squamous cell carcinoma, well differentiated skin, lower leg T3N0M0
22 48 M squamous cell carcinoma, well differentiated skin, heel TXNXMX
23 67 M squamous cell carcinoma  skin, leg T3N0M0
24 80 F squamous cell carcinoma well differentiated skin, shoulder T1N0M0
25 70 M squamous cell carcinoma, poorly differentiated skin, abdomen TXNXM1
*26 66 M squamous cell carcinoma, moderately differentiated skin, neck 3.5 cm
*27 61 M metastatic squamous cell carcinoma skin, chest wall 6 cm
28 42 M recurrent squamous cell carcinoma skin, neck T4N1M0
29 50 M squamous cell carcinoma, well differentiated skin, lower leg T4N0M0
30 41 M recurrent squamous cell carcinoma skin, inguinal T3NXMX
31 38 M squamous cell carcinoma, well differentiated skin, toe T4N0M0
*32 48 M recurrent squamous cell carcinoma skin, neck 3.2 cm
*33 54 M recurrent squamous cell carcinoma skin, neck 3.5 cm
34 42 M recurrent squamous cell carcinoma unkown T2N1M0
35 63 M squamous cell carcinoma, moderately differentiated skin, chest wall T4N1M1
*36 68 M metastatic squamous cell carcinoma skin, chest wall 1.5 cm
*37 64 F metastatic squamous cell carcinoma skin, abdomen 2 cm
38 55 M squamous cell carcinoma, well differentiated ear T2N1M0
39 42 M squamous cell carcinoma, well differentiated tongue T2N1M0
*40 58 M metastatic squamous cell carcinoma skin, neck 10 cm
41 75 M squamous cell carcinoma, well differentiated larynx 2.5 cm
*42 45 M squamous cell carcinoma, moderately differentiated skin, neck 2.5 cm
43 40 M squamous cell carcinoma, poorly differentiated skin, scalp T2N0M0
*44 72 M squamous cell carcinoma, well differentiated lung 3.5 cm
45 65 M squamous cell carcinoma, well differentiated skin, thigh T3N0M0
*46 66 M recurrent squamous cell carcinoma skin, neck 1 cm
47 80 F squamous cell carcinoma, well differentiated skin, buttock T2N0M0
48 73 F squamous cell carcinoma skin, wrist T1N0M0
49 47 M squamous cell carcinoma well differentiated skin, heel T2N0M0
50 74 M adenoid cystic carcinoma skin, axilla 3.5 cm
51 Normal of No. 1  
 
   
52 Normal of No. 7        
53 Normal of No. 8        
54 Normal of No. 20        
55 Normal of No. 38        
56 Normal of No. 39        
57 Normal of No. 42        
58 Normal of No. 43        
59 Normal of No. 45        
60 carbon particle        
           

General Information of the Tissue Array Slide :

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

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Published in Cell culture
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