Price : 5 slide for 600 $

CX1  Human, skin cancer
	Age	Sex	Diagnosis	Site	Stage
1	53	M	squamous cell carcinoma, well differentiated	skin, face	T2N0M0
2	54	M	squamous cell carcinoma, well differentiated	skin, scalp	T3N0M0
3	62	M	squamous cell carcinoma, well differentiated	skin, neck	T2N0M0
4	58	F	squamous cell carcinoma, well differentiated	skin, foot	TXNXMX
5	55	M	squamous cell carcinoma, moderately differentiated	skin neck	T4N0M0
6	60	M	squamous cell carcinoma, moderately differentiated	skin, face	T2N0M0
*7	57	M	squamous cell carcinoma, moderately differentiated	pharynx	T4N0M0
8	61	M	squamous cell carcinoma, well differentiated	skin elbow	T4N1M0
*9	25	F	metastatic squamous cell carcinoma	skin, scalp
10	67	M	squamous cell carcinoma, well differentiated	skin, neck	T4N0M0
11	63	M	squamous cell carcinoma, poorly differentiated	skin, perianal	T2NXM0
12	63	M	recurrent squamous cell carcinoma	skin, face	T4NXM0
*13	87	F	squamous cell carcinoma, well differentiated	skin face	T1N0M0
*14	66	M	squamous cell carcinoma, poorly differentiated	skin, neck	T4NXM0
15	48	M	squamous cell carcinoma	skin, axilla	T4N12M1
16	65	M	squamous cell carcinoma	anus	TXNXMX
*17	67	M	metastatic squamous cell carcinoma	skin, chest wall	3.5 cm
18	48	M	squamous cell carcinoma, moderately differentiated	skin, lower leg	T3N0M0
19	51	F	squamous cell carcinoma, well differentiated	skin, thigh	T3N0M0
20	40	M	squamous cell carcinoma, well differentiated	skin, knee	T4N0M0
21	40	M	squamous cell carcinoma, well differentiated	skin, lower leg	T3N0M0
22	48	M	squamous cell carcinoma, well differentiated	skin, heel	TXNXMX
23	67	M	squamous cell carcinoma	skin, leg	T3N0M0
24	80	F	squamous cell carcinoma well differentiated	skin, shoulder	T1N0M0
25	70	M	squamous cell carcinoma, poorly differentiated	skin, abdomen	TXNXM1
*26	66	M	squamous cell carcinoma, moderately differentiated	skin, neck	3.5 cm
*27	61	M	metastatic squamous cell carcinoma	skin, chest wall	6 cm
28	42	M	recurrent squamous cell carcinoma	skin, neck	T4N1M0
29	50	M	squamous cell carcinoma, well differentiated	skin, lower leg	T4N0M0
30	41	M	recurrent squamous cell carcinoma	skin, inguinal	T3NXMX
31	38	M	squamous cell carcinoma, well differentiated	skin, toe	T4N0M0
*32	48	M	recurrent squamous cell carcinoma	skin, neck	3.2 cm
*33	54	M	recurrent squamous cell carcinoma	skin, neck	3.5 cm
34	42	M	recurrent squamous cell carcinoma	unkown	T2N1M0
35	63	M	squamous cell carcinoma, moderately differentiated	skin, chest wall	T4N1M1
*36	68	M	metastatic squamous cell carcinoma	skin, chest wall	1.5 cm
*37	64	F	metastatic squamous cell carcinoma	skin, abdomen	2 cm
38	55	M	squamous cell carcinoma, well differentiated	ear	T2N1M0
39	42	M	squamous cell carcinoma, well differentiated	tongue	T2N1M0
*40	58	M	metastatic squamous cell carcinoma	skin, neck	10 cm
41	75	M	squamous cell carcinoma, well differentiated	larynx	2.5 cm
*42	45	M	squamous cell carcinoma, moderately differentiated	skin, neck	2.5 cm
43	40	M	squamous cell carcinoma, poorly differentiated	skin, scalp	T2N0M0
*44	72	M	squamous cell carcinoma, well differentiated	lung	3.5 cm
45	65	M	squamous cell carcinoma, well differentiated	skin, thigh	T3N0M0
*46	66	M	recurrent squamous cell carcinoma	skin, neck	1 cm
47	80	F	squamous cell carcinoma, well differentiated	skin, buttock	T2N0M0
48	73	F	squamous cell carcinoma	skin, wrist	T1N0M0
49	47	M	squamous cell carcinoma well differentiated	skin, heel	T2N0M0
50	74	M	adenoid cystic carcinoma	skin, axilla	3.5 cm
51	Normal of No. 1
52	Normal of No. 7
53	Normal of No. 8
54	Normal of No. 20
55	Normal of No. 38
56	Normal of No. 39
57	Normal of No. 42
58	Normal of No. 43
59	Normal of No. 45
60	carbon particle

General Information of the Tissue Array Slide :

Applications

• Immunohistochemistry
• In situ hybridization, RNA or DNA
• Fluorescent in situ hybridization (FISH)
• In situ PCR, RNA or DNA
• TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

• Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
• Tissue array slides are shipped in room temperature.
• If the slides are stored in 4 degree, they are good up to one year.

How processed

• Tissues were initially fixed with formalin except for some of the animal tissues.
• Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
• Cleared by xylene, three changes for 1 hour each.
• Infiltrated with 60°C paraffin, three changes for 1 hour each
• Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

• Dry slides for 1 hour in a oven at 60 degree.
• Dewax slides in xylene for 4 minutes x 5 times
• Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
• Immerse slides in tap water for 5 minutes

Slide orientation

• In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.