Non-neoplastic liver tissue

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Category Human tissue array
   Cat. No. CSN
   Product description Non-neoplastic liver tissue
   Data sheet CSN1
CSN1
  general_information
   No. of samples 59
   Text
Current version: CSN1 
Non-neoplastic liver: 59 cores 
matching with CS1

Price : 5 slide for 600 $

CSN1 Human, non-neoplastic liver
           
  Age Sex Diagnosis Patients' major problem No. of CS1#
101 68 M reactive hepatitis rectum adenocarcinoma  
102 60 M reactive hepatitis rectum adenocarcinoma  
103 63 F nonspecific reactive change colon adenocarcinoma  
104 57 M chronic persistent hepatitis HCC 2
105 47 M nonspecific reactive change rectum adenocarcinoma  
106 50 M reactive hepatitis stomach adenocarcinoma  
107 65 F nonspecific reactive change colon adenocarcinoma  
108 43 F reactive hepatitis gallbladder cancer  
109 48 M reactive hepatitis with steatosis gallbladder cancer  
110 67 M reactive hepatitis colon adenocarcinoma  
111 59 M reactive hepatitis colon adenocarcinoma  
112 52 M chronic active hepatitis HCC 7
113 60 M reactive hepatitis gallbladder cancer  
114 52 F reactive hepatitis gallbladder cancer  
115 58 M reactive hepatitis with cholestasis common bile duct carcinoma  
116 48 F reactive hepatitis stomach adenocarcinoma  
117 62 F nonspecific reactive change colon adenocarcinoma  
118 52 M nonspecific reactive change colon adenocarcinoma  
119 73 F reactive hepatitis with cholestasis gallbladder cancer  
120 30 M reactive hepatitis with cholestasis common bile duct cancer  
121 45 M chronic active hepatitis colon adenocarcinoma  
122 71 M reactive hepatitis rectum adenocarcinoma  
123 61 M reactive hepatitis stomach adenocarcinoma  
124 71 M nonspecific reactive change lung adenocarcinoma  
125 59 M nonspecific reactive change colon adenocarcinoma  
126 67 M reactive hepatitis cholangiocarcinoma  
127 62 M nonspecific reactive change colon adenocarcinoma  
128 52 M nonspecific reactive change with steatosis rectum adenocarcinoma  
129 74 F nonspecific reactive change with steatosis colon adenocarcinoma  
130 81 M nonspecific reactive change common bile duct cancer  
131 37 M chronic persistent hepatitis HCC 14
132 75 M macronodular cirrhosis HCC 15
133 32 M macro- and micronodular cihhrosis combined HCC & cholangiocarcinoma 17
134 57 M micronodular cirrhosis HCC 19
135 65 M chronic persistent hepatitis HCC 22
136 52 M macro- and micronodular cihhrosis HCC 23
137 65 M macronodular cirrhosis HCC 24
138 72 M reactive hepatitis HCC 25
139 58 F macro- and micronodular cihhrosis HCC 26
140 52 M micronodular cirrhosis HCC 27
141 40 M macro- and micronodular cihhrosis HCC 28
142 53 F macro- and micronodular cihhrosis HCC 29
143 67 M macro- and micronodular cihhrosis HCC 30
144 47 M reactive hepatitis HCC 34
145 65 M dysplastic nodule, high grade HCC 37
146 63 F macro- and micronodular cihhrosis HCC 38
147 60 F macronodular cirrhosis HCC 39
148 65 F macronodular cirrhosis HCC 40
149 66 F micronodular cirrhosis HCC 42
150 65 F macronodular cirrhosis HCC 43
151 64 M macro- and micronodular cihhrosis HCC 46
152 51 M macro- and micronodular cihhrosis HCC 47
153 65 F macro- and micronodular cihhrosis HCC 48
154 51 M chronic persistent hepatitis HCC 52
155 49 M chronic persistent hepatitis HCC 53
156 57 F chronic persistent hepatitis HCC 54
157 57 M reactive hepatitis HCC 55
158 50 M reactive hepatitis HCC 57
159 43 M micronodular cirrhosis HCC 58
160 carbon        
HCC : hepatocellular carcinoma      
#: Cancer tissue in CS1 of corresponding number is from the identical patient.    

General Information of the Tissue Array Slide :

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
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