Cellulose Filters

Qualitative filter papers

Cellulose filters
> Qualitative filters
Qualitative filter papers
These cellulose filters are used in qualitative analytical techniques to determine and identify materials. Prepleated qualitative filters are also available, which give improved flow rate and increase loading capacity compared to equivalent flat filters. 

In addition, Whatman offers wet strengthened qualitative filters which contain a small quantity of a chemically stable resin to give improved high wet strength. This does not introduce any significant impurities into the filtrate. The resin, however, does contain nitrogen so these grades should not be used in Kjedldahl. All wet-strengthened grades are available in prepleated forms. Whatman provides a wide range of Qualitative Filters to meet your specific needs.

Qualitative Filter Papers - Standard Grades

Grade 1: 11µm
The most widely used filter paper for routine applications with medium retention and flow rate. Extended range of sizes includes 10mm to 500mm diameter circles and 460mm x 570mm sheets. This filter is also available in the Whatman FilterCup™. This is a convenient, disposable 70mm filter funnel with a 250mL capacity moulded from polypropylene with an integral, heat bonded filter.

This grade covers a wide range of laboratory applications and is frequently used for clarifying liquids. Traditionally the grade is used in qualitative analytical separations for precipitates such as lead sulphate, calcium oxalate (hot) and calcium carbonate.

In agriculture, it is used for soil analysis and seed testing procedures. In the food industry, Grade 1 is used for numerous routine techniques to separate solid foodstuffs from associated liquid or extracting liquid and is widely used in education for teaching simple qualitative analytical separations.

In air pollution monitoring, using circles or rolls, atmospheric dust is collected from airflow and the stain-intensity measured photometrically. For gas detection, the paper is impregnated with a chromogenic reagent and colour formation quantified by optical reflectance.

Grade 2: 8µm 
Slightly more retentive than Grade 1 with a corresponding increase in filtration time (i.e., slightly slower filtration speed). More absorbent than Grade 1. Also available in folded (prepleated) form as Grade 2V. In addition to general filtration in the 8µm particle size range, the extra absorbency is utilized, for example, to hold soil nutrient in plant growth trials. Also used for monitoring specific contaminants in the atmosphere and in soil testing.

Grade 3: 6µm 
Double the thickness of Grade 1 with still finer particle retention and excellent loading capacity; more precipitate can be held without clogging. The extra thickness gives increased wet strength and makes this grade highly suitable for use in Büchner funnels. The high absorbency is particularly valuable when the paper is used as a sample carrier. This filter is also available in the Whatman FilterCup. This is a convenient, disposable 70mm filter funnel with a 250mL capacity moulded from polypropylene with an integral, heat bonded filter.

Grade 4: 20-25µm 
Extremely fast filtering with excellent retention of coarse particles and gelatinous precipitates such as ferric hydroxide and aluminium hydroxide. Very useful as a rapid filter for routine cleanup of biological fluids or organic extracts during analysis. Used when high flow rates in air pollution monitoring are required and the collection of fine particles is not critical.

Grade 5: 2.5µm 
The maximum degree of fine particle filtration in the qualitative range. Capable of retaining the fine precipitates encountered in chemical analysis. Slow flow rate. Excellent clarifying filter for cloudy suspensions and for water and soil analysis.

Grade 6: 3µm 
Twice as fast as Grade 5 with similar fine particle retention. Often specified for boiler water analysis applications.

Cellulose filters
> Wet Strengthened Filters

Qualitative Filter Papers - Wet Strengthened Grades

These extremely strong filter papers have a high wet strength due to the addition of a small quantity of chemically stable resin. Normal qualitative applications will not introduce any significant impurities into the filtrate. The resins do, however, contain nitrogen so these grades should not be used in Kjeldahl estimations, etc. All wet-strengthened grades are available in folded (prepleated) forms.

Grade 91: 10µm
A general-purpose crêped filter for less critical routine analysis. Widely used to assay sucrose in cane sugar and within pharmaceutical laboratories for routine filtration.

Grade 113: 30µm Ultra high loading capacity with a particle retention making it ideal for use with coarse or gelatinous precipitates. Fastest flow rate of the qualitative grades. Creped surface. Thickest filter paper in the Whatman range. This filter is also available in the Whatman FilterCup. This is a convenient, disposable 70mm filter funnel with a 250mL capacity moulded from polypropylene with an integral, heat bonded filter.

Grade 114: 25µm
Only half the thickness of Grade 113 and suitable for coarse or gelatinous precipitates. Smooth surface for easy recovery of precipitates.

Cellulose filters
> Prepleated Filters

Qualitative Filter Papers - Folded (Prepleated Grades)

Time-saving Whatman qualitative grades are offered in this convenient form, which have major advantages over flat circles:

  • Savings in time required to quadrant-fold circles to fit conical filter funnels in repetitive or multiple analysis
  • Decreased overall filtration time because of the extra surface area exposed; the normal slow down of filtration speed due to the loading of particulate is postponed
  • Increased total loading capacity because more filter area is available
  • Maintained flow rate because of the reduction in filter paper contact with funnel side and, of course, the self-supporting shape of the filter itself
  • The prepleating does not significantly affect any of the technical data and the same figures may be used for the flat circles.

Grade 2V: 8µm
Widely used for general purpose filtration. Slightly more retentive than No. 1. Has excellent particle retention and a good filtration speed and loading capacity.

Grade 113V: 30µm
Very thick and strong filter with creped surface for extremely high loading capacity, particularly in folded form. Fastest flow rate of any qualitative grade. Ideal for coarse particles and gelatinous precipitates.

Grade 114V: 25µm
Strong filter with very fast flow rate. Ideal for coarse particles and gelatinous precipitates. Smooth surface.

Qualitative Filter Papers - Student Grade/Grade 93

Designed for educational use, this filter paper is intermediate in speed and retention between Grades 1 and 4. Student grade retains those flocculent and medium precipitates most often encountered in student experiments.

Available in a dispenser pack, it can be attached to the wall or bench, placed on a shelf either upright or flat, and used as a normal carton or as a convenient dispenser. The envelopes are released individually for easy one-at-a-time removal. Package and envelopes are clearly marked for size and content.

Standard Circle Filter Funnel Volumes
The maximum practical volume of the most popular disc sizes (quadrant folded) are:
Diameter (mm) Volume (mL)
90 15
110 20
125 35
150 75
185 135
240 300
Ordering Information - Qualitative Standard Filter Circles
  Catalogue Number  
Diameter (mm) Grade 1 Grade 2 Grade 3 Grade 4 Grade 5 Grade 6 Quantity/Pack
10 1001-6508 — — — — — 500
23 — — 1003-323 — — — 100
25 1001-325 — — 1004-325 1005-325 — 100
30 1001-329 — — — — — 100
32 1001-032 — — — — — 100
42.5 1001-042 1002-042 1003-042 1004-042 1005-042 1006-042 100
47 1001-047 — — 1004-047 1005-047 — 100
55 1001-055 1002-055 1003-055 1004-055 1005-055 — 100
70 1001-070 1002-070 1003-070 1004-070 1005-070 1006-070 100
85 1001-085 — — — — — 100
90 1001-090 1002-090 1003-090 1004-090 1005-090 1006-090 100
110 1001-110 1002-110 1003-110 1004-110 1005-110 1006-110 100
125 1001-125 1002-125 1003-125 1004-125 1005-125 1006-125 100
150 1001-150 1002-150 1003-150 1004-150 1005-150 1006-150 100
185 1001-185 1002-185 1003-185 1004-185 1006-185 1006-185 100
240 1001-240 1002-240 1003-240 1004-240 1006-240 1006-240 100
270 1001-270 1002-270 — 1004-270 — — 100
320 1001-320 1002-320 1003-320 1004-320 1005-320 — 100
385 1001-385 1002-385 — — — — 100
400 1001-400 — — 1004-400 — — 100
500 1001-500 1002-500 1003-500 — — — 100
FilterCup 70**** 1600-001 — 1600-003 — — — 25
Ordering Information - Qualitative Wet Strengthened Filter Circles
  Catalogue Number  
Diameter (mm) Grade 91 Grade 93/Student Grade 113 Grade 114 Quantity/Pack
90 — — 1113-090 1114-090 100
110 1091-110* 1093-111** 1113-110 — 100
125 1091-125* 1093-126** 1113-125 1114-125 100
150 1091-150*** — 1113-150 1114-150 100
185 1091-185*** — 1113-185 1114-185 100
190 1091-190 — — — 100
240 1091-240*** — 1113-240 1114-240 100
270 — — — 1114-270 100
320 — — 1113-320 — 100
400 — — — 1114-400 100
500 — — 1113-500 — 100
FilterCup 70**** — — 1600-113 — 25

* Packed in 4000 subdivided into 100. 
** Packed in 50 envelopes of 25 circles each. 
*** Packed in 1000 subdivided into 100. 
**** FilterCup Stem with Stopper - one time purchase - Catalogue Number 1600-900
Ordering Information - Qualitative Filter Circles Folded (pleated)
  Catalogue Number  
Diameter (mm) Grade 2V Grade 113V Grade 114V Quantity/Pack
125 1202-125 1213-125 1214-125 100
150 1202-150 1213-150 1214-150 100
185 1202-185 1213-185 1214-185 100
240 1202-240 1213-240 1214-240 100
270 1202-270 1213-270 — 100
320 1202-320 1213-320 1214-320 100
385 1202-385 — — 100
400 1202-400 — — 100
500 1202-500 1213-500 — 100
Ordering Information - Qualitative Standard Filter Sheets
Dimensions (mm) Catalogue Number Quantity/Pack
Grade 1
460 x 570 1001-917 100
460 x 570 1001-918 500
580 x 680 1001-931 100
Grade 2
460 x 570 1002-917 100
580 x 680 1002-931 100
Grade 3
460 x 570 1003-917 100
Grade 4
460 x 570 1004-917 100
Ordering Information - Qualitative Filter Paper
  Catalogue Number  
Diameter (mm) Grade 595 Grade 597 Grade 598 Grade 602 h Quantity/Pack
12.7 — 10 311 862 — — 1000
42.5 — 10 312 040 — — 100
45 — 10 311 804 — — 100
55 — 10 311 807 — — 100
70 — 10 311 808 — — 100
90 — 10 311 809 10 312 209 10 312 609 100
110 10 311 610 10 311 810 — — 100
125 10 311 611 10 311 811 — 10 312 611 100
150 10 311 612 10 311 812 — 10 312 612 100
185 — 10 311 814 — 10 312 614 100
240 — 10 311 820 — 10 312 620 100
320 — 10 311 822 — — 100
Diameter (mm) Grade 520 a Grade 2294 Grde 2589 a   Quantity/Pack
110 — 10 342 810 —   100
140 — — 10 343 630   500
180 / 33 ZL1 — 10 342 860 —   100
210 / 60 ZL1 — 10 342 862 —   100
270 10 331 421 — —   100
Diameter (mm) Grade 0048 Grade 551 Grade 3612   Quantity/Pack
32 10 348 903 — —   1000
90 — 10 310 809 —   100
240 — — 10 341 420   100
1Round filter with central hole
Ordering Information - Qualitative Folded (pleated) Filter Papers
  Catalogue Number  
Diameter (mm) Grade 520 a ½ Grade 595 ½ Grade 597 ½ Grade 602 h ½ Grade 2555 ½ Quantity/Pack
70 — — 10 311 841 — — 100
90 — — 10 311 842 10 312 642 — 100
110 — 10 311 643 10 311 843 — — 100
125 — 10 311 644 10 311 844 10 312 644 — 100
150 — 10 311 645 10 311 845 10 312 645 — 100
185 — 10 311 647 10 311 847 10 312 647 10 313 947 100
240 10 331 451 10 311 651 10 311 851 10 312 651 10 313 951 100
270 — 10 311 652 10 311 852 — — 100
320 — 10 311 653 10 311 853 — 10 313 953 100
385 — — 10 311 854 — — 100
500 10 331 456 — 10 311 856 — — 100
700 10 331 459 — — — — 100
Diameter (mm) Grade 591 ½ Grade 520 b II ½ Grade 598 ½ Grade 287 ½   Quantity/Pack
125 — — 10 312 244 10 310 244   50
150 — — — 10 310 245   50
185 10 311 347 — 10 312 247 10 310 247   50
240 10 311 351 — 10 312 251 10 310 251   50
320 — 10 331 653 — 10 310 253   50
500 — — 10 312 256 —   50
Ordering Information - Qualitative Filter Paper Sheets
  Catalogue Number  
Dimensions (mm) Grade 591 Grade 595 Grade 597 Grade 598 Grade 520 a Grade 520 bII Quantity/ Pack
580 x 580 — — 10 311 897 — — — 100
580 x 580 10 311 387 — — 10 312 287 10 331 487 10 331 681 250
580 x 580 — 10 311 687 10 311 887 — — — 500
Dimensions (mm) Grade 2411 Grade 2589 a Grade 2589 c Grade 2589 d Grade 0860   Quantity/Pack
580 x 580 — 10 343 687 — — 10 334 597   100
580 x 580 10 343 287 — — — —   200
25 x 75 — — 10 343 876 10 343 976 —   100
Dimensions (mm) Grade 0903 Grade 0905         Quantity/Pack
580 x 580 10 334 887 10 334 987         500
450 x 450 10 334 885 10 334 985         500
Ordering Information - Shark Skin Filters
Diameter (mm) Catalogue Number Quantity/Pack
110 10 347 510 100
150 10 347 513 100
290 10 347 577 100
320 10 347 530 100
812.8 10 347 576 100
Ordering Information - Shark Skin Filter Sheets
Dimensions (inches) Catalogue Number Quantity/Pack
8 x 10 10 538 877 100
26 x 53 10 347 588 500
32 x 64 10 347 530 500
37 x 37 10 538 873 500
21 x 750 feet 10 537 138 1
 
 
 

Quantitative filter papers

Cellulose filters
> Quantitative filters
Quantitative filter papers
Whatman quantitative filters are designed for gravimetric analysis and the preparation of samples for instrumental analysis. They are available in three formats designed to meet your specific needs. 
  • Ashless: 0.007% ash maximum - very pure filters ideal for a wide range of critical analytical filtration procedures.
  • Hardened low ash: 0.015% ash maximum - treated with a strong acid to remove trace metals and produce high wet strength and chemical resistance. These filters are particularly suitable for Büchner filtration where the tough smooth surface of the filter makes it easy to recover precipitates.
  • Hardened ashless: 0.006% ash maximum - acid hardened to give high wet strength and chemical resistance with extreme low ash content. The tough surface makes these filters suitable for a wide range of critical filtration procedures.

Quantitative Filter Papers - Ashless Grades (Ash 0.007%)

Grade 40: 8µm
The classic general purpose ashless filter paper with medium speed and retention. Typical applications include gravimetric analysis for numerous components in cements, clays, iron and steel products; as a primary filter for separating solid matter from aqueous extracts in general soil analysis, quantitative determination of sediments in milk and as a pure analytical grade clean-up filter for solutions prior to AA spectro- photometry. Used also as a high purity filter in the collection of trace elements and radionuclides from the atmosphere.

Grade 41: 20-25µm 
The fastest ashless filter paper, recommended for analytical procedures involving coarse particles or gelatinous precipitates (e.g., iron or aluminium hydroxides). Also used in quantitative air pollution analysis as a paper tape for impregnation when determining gaseous compounds at high flow rates. This filter is also available in the Whatman Disposable Filter Funnel. This is a convenient, disposable 47mm filter funnel with a 250mL capacity. The 47mm Grade 41 filter can be easily removed for further analysis or culturing.

Grade 42: 2.5µm 
A world standard for critical gravimetric analysis with the finest particle retention of all Whatman cellulose filter papers. Typical analytical precipitates include barium sulphate, metastannic acid and finely precipitated calcium carbonate.

Grade 43: 16µm 
Intermediate in retention between Grade 40 and 41, and twice as fast as Grade 40. Typical applications include foodstuffs analysis; soil analysis; particle collection in air pollution monitoring for subsequent analysis by XRF techniques; and inorganic analysis in the construction, mining and steel industries.

Grade 44: 3µm 
Thin version of Grade 42 retaining very fine particles but with lower ash weight per sample and almost twice the flow rate as Grade 42.

Cellulose filters
> Quantitative hardened filters

Quantitative Filter Papers - Hardened Low Ash Grades (Ash 0.015%)

The maximum ash content of these grades is intermediate between ashless and qualitative grades. They are particularly suitable for Buchner filtrations where it is desirable to recover the precipitate from the filter surface after filtration. Other characteristics include high wet strength and chemical resistance which are similar to the acid hardened ashless filter papers.

Grade 50: 2.7µm 
Retention of finest crystalline precipitates. The thinnest of all Whatman filter papers. Slow flow rate. Hardened and highly glazed surface. This finish also keeps the paper free from loose surface fibres. Highly suitable for qualitative or quantitative filtrations requiring vacuum assistance on Büchner or 3-piece filter funnels. Very strong when wet. Will withstand wet handling and precipitate removal by scraping. In the electronics industry, the virtual absence of fibre shedding is utilised in carriers for integrated circuits.

Grade 52: 7µm 
The general purpose hardened filter paper with medium retention and flow rate. Very hard surface.

Grade 54: 20-25µm
Very fast filtration for use with coarse and gelatinous precipitates. High wet strength makes this grade very suitable for vacuum assisted fast filtration of "difficult" coarse or gelatinous precipitates.

Quantitative Filter Papers - Hardened Ashless Grades (Ash 0.006%)

These are the supreme quantitative filter papers featuring high wet strength and chemical resistance. These papers are acid hardened, which reduces ash to an extremely low level. Their tough surfaces make them suitable for a wide range of critical analytical filtration operations. Each grade offers a convenient combination of filtration speed and particle retention.

Grade 540: 8µm 
The general purpose hardened ashless filter paper with medium retention and flow rate. Extremely pure and strong with a hard surface. High chemical resistance to strong acid and alkali. Frequently used in the gravimetric analysis of metals in acid/alkali solutions and in collecting hydroxides after precipitation by strong alkalis.

Grade 541: 20-25µm 
Fast filtration of coarse particles and gelatinous precipitates in acid/alkali solutions during gravimetric analysis. Typical applications include fibre in animal foodstuffs, gelatine in milk and cream, chloride in cement, and chloride and phosphorous in coal and coke.

Grade 542: 2.7µm
High retention of fine particles under demanding conditions. Slow flow rate. Very hard and strong with excellent chemical resistance. Often used in gravimetric metal determinations.

Standard Circle Filter Funnel Volumes
The maximum practical volume of the most popular disc sizes (quadrant folded) are:
Diameter (mm) Volume (mL)
90 15
110 20
125 35
150 75
185 135
240 300
Ordering Information - Quantitative Ashless Filter Circles
  Catalogue Number  
Diameter (mm) Grade 40 Grade 41 Grade 42 Grade 43 Grade 44 Quantity/Pack
Filter Circles
30 1440-329 — — — — 100
42.5 1440-042 1441-042 1442-042 — — 100
47 1440-047 1441-047 1442-047 — — 100
55 1440-055 1441-055 1442-055 — — 100
70 1440-070 1441-070 1442-070 — 1444-070 100
90 1440-090 1441-090 1442-090 1443-090 1444-090 100
110 1440-110 1441-110 1442-110 1443-110 1444-110 100
125 1440-125 1441-125 1442-125 1443-125 1444-125 100
150 1440-150 1441-150 1442-150 1443-150 1444-150 100
185 1440-185 1441-185 1442-185 1443-185 1444-185 100
240 1440-240 1441-240 1442-240 — — 100
320 — 1441-320 1442-320 — — 100
Disposable Filter Funnel
    1920-1441       5
Filter Sheets
8" x 10" — 1441-866 — — — 100
460 mm x 570 mm   1441-917 — — — 100
Ordering Information
Quantitative Hardened Low Ash Filter Circles Quantitative Hardened Ashless Filter Circles
  Catalogue Number Catalogue Number  
Diameter (mm) Grade 50 Grade 52 Grade 54 Grade 540 Grade 541 Grade 542 Quantity/Pack
21 — — — 1540-321 — — 100
24 — — — 1540-324 — — 100
42.5 1450-042 — — 1540-042 1541-042 — 100
47 — — — — 1541-047 — 100
55 1450-055 — 1454-055 1540-055 1541-055 1542-055 100
70 1450-070 1452-070 1454-070 1540-070 1541-070 1542-070 100
90 1450-090 1452-090 1454-090 1540-090 1541-090 1542-090 100
110 1450-110 1452-110 1454-110 1540-110 1541-110 1542-110 100
125 1450-125 1452-125 1454-125 1540-125 1541-125 1542-125 100
150 1450-150 1452-150 1454-150 1540-150 1541-150 1542-150 100
185 1450-185 — 1454-185 1540-185 1541-185 1542-185 100
240 1450-240 1452-240 1454-240 1540-240 1541-240 1542-240 100
320 1450-320 — 1454-320 — 1541-320 — 100
400 — — — — 1541-400 — 100
500 — — 1454-500 — — — 100
Surface Wipes
Smear Tab 1450-993 — — — — — 100
Filter Sheets
460 mm x 570 mm — — 1454-917 — 1541-917 — 100
Ordering Information - Quantitative Filter Paper
  Catalogue Number  
Diameter (mm) Grade 589/1 Grade 589/2 Grade 589/3 Quantity/Pack
12.7 — 10 300 102 — 1000
40.5 — 10 300 103 — 100
50 — 10 300 106 — 100
55 — 10 300 107 — 100
70 — 10 300 108 — 100
90 10 300 009 10 300 109 — 100
110 10 300 010 10 300 110 10 300 210 100
125 10 300 011 10 300 111 10 300 211 100
150 10 300 012 10 300 112 10 300 212 100
185 10 300 014 10 300 114 10 300 214 100
240 — 10 300 120 — 100

Cellulose filters

Ordering Information - Quantitative Folded (pleated) Filter Papers
  Catalogue Number  
Diameter (mm) Grade 589/2 ½ * Quantity/Pack
110 10 300 143 100
150 10 300 145 100
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Extraction Products

Whatman offers a unique line of high quality extraction products to meet a wide range of extraction applications. These products are manufactured from high-alpha cellulose cotton linters for purity and consistent performance.
 

Whatman 1 PS Phase Separator

Separator paper
Whatman 1 PS Phase Separator 
The Whatman 1 PS Phase Separator is a high grade filter paper impregnated with a stabilised silicone that renders it hydrophobic, retaining the aqueous phase and passing the solvent phase through. 

Automatic Cut-Off, Separatory Funnel Replacement
After being shaken, the mixed phases are simply poured directly into the 1 PS circle which is quadrant-folded in the funnel. The separation is extremely rapid so it is unnecessary to wait until the two phases have settled into separate layers. Droplets are automatically separated after only a few moments, giving a solvent phase completely free of the aqueous phase.

In many applications, 1 PS can replace the use of separatory funnels. The solvent phase flows through the paper quickly and cleanly. It then stops automatically, leaving the aqueous phase completely in the paper. This feature is particularly important when carrying out a large number of routine solvent extractions at the same time. Samples can be shaken with solvent in stoppered conical flasks or test tubes and transferred directly to funnels containing 1 PS.

Unsupervised Separation
A chief benefit of the 1 PS method is that cut-off is automatic and complete just as soon as the solvent phase has passed through. The result is no skilled operators are required.

Features and Benefits

  • Ease of use. No special training required
  • Any number of separations can proceed together
  • Staff involvement in routine separations is at a minimum

Safety 
Water may break through upon prolonged standing.

Ordering Information - 1PS Phase Separators (Circles)
Catalogue Number Diameter Quantity/Pack
2200-070 7 100
2200-090 9 100
2200-110 11 100
2200-125 12.5 100
2200-150 15 100
2200-185 18.5 100
2200-240 24 100
2200-270 27 100
 
 
 
Solid phase extraction (SPE)
 

Whatman SPE devices are designed to concentrate or isolate analytes from complex sample matrices. Available with a variety of packing media, these devices offer the advantage of working with different types of interactions between the sample components, sorbent and suitable eluent. The polarity (polar, nonpolar) or charge (anion, cation) of the analyte of interest will determine the proper choice of sorbent and solvent.

Whatman SPE devices have silica-based chemistries and are available in several configurations. Column capacities include 3, 6 and 12 mL size columns. A cartridge format for use with a syringe is also available.

Solid phase extraction is a chromatographic technique used to prepare samples for subsequent analysis by removing interfering substances that may be present. This is done either by retaining the substance of interest and washing off everything else or by retaining the interfering substances and eluting the product of interest.

Whatman SPE devices contain high quality sorbets for LC chromatographers.

Features and Benefits

  • Available in a range of packing media
  • Whatman quality sorbents for consistent results

Applications

  • Isolate analytes from complex sample matrices
  • Remove interfering substances in order to prepare samples for subsequent analysis
  • Drug metabolites in biological fluids
  • Food analysis
  • Environmental analysis

Solid Phase Extraction (SPE) Disks

The Whatman SPE disk utilizes C-18 derivatized silica, incorporated into a glass microfiber matrix. The high flow and high loading capacity of the glass microfiber media allow for rapid aqueous sample flow rates, while oil, grease and other organic analytes are efficiently extracted and retained by the reverse phase silica material.

Features and Benefits

  • High quality Whatman glass microfiber media for superior flow and high loading capacity
  • Efficient grease analyte extraction and retention

Applications

  • Oil and grease analysis, EPA Method 1664A

Sample Drying Device

A sample drying device is available for removal of water from organic extracts. It attaches to the male luer outlet of a syringe in which the organic sample extract has been collected. Traces of water are removed as the sample is pushed through the drying device into a collection vial.

Typical Data
Key to Sorbent Abbreviations Features
ODS-4 Octadecyl silane 14% of carbon load, end capped
ODS-5 Octadecyl silane 18% of carbon load, end capped
C-8 Octyl silane 8.5% of carbon load, end capped
FLO Florisil® —
SCX Strong cation exchanger–  
  aromatic benzene sulfonic acid —
  functional groups  
SAX Strong anion exchanger–  
  quaternary amino groups (-NR3+) —
Ordering Information + Solid Phase Extraction
Catalogue Number Product Code Column Volume Quantity/Pack
Column Type
6803-0505 ODS-5 500 mg/3 mL 50
6803-0507 ODS-5 500 mg/6 mL 30
6803-0509 ODS-5 1000 mg/12 mL 20
6803-1205 C-8 500 mg/3 mL 50
6803-1809 FLO 1000 mg/12 mL 20
6803-2005 SAX 500 mg/3 mL 50
6803-2605 SCX 500 mg/3 mL 50
Cartridge Type*
6804-0405 ODS-4 500 mg/unit 50
6804-0505 ODS-5 500 mg/unit 5
Ordering Information - Solid Phase Extraction (SPE) Disks
Catalogue Number Description Filter Media Diameter (mm) Pore Size (µm) Quantity/Box
6805-3402 SPE disk for oil and grease — 47 — 20
6805-3043 SPE disk for oil and grease — 47 — 80
6805-3048 SPE disk for oil and grease — 90 — 20
6805-8034 DFP prefilter — 42.5 5 48
6805-8035 DFP prefilter for 47 mm disk PP 47 5 48
6805-8037 DFP prefilter for 90 mm disk PP 90 5 16
Ordering Information - Sample Drying Device
Cartridge Type with Polypropylene Filter
Catalogue Number Product Size (mg) Quantity/Pack
6805-8020 Sodium sulfate with 0.45 µm - PP filter and tube tip 1500 50
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Chromatography cellulose media

 

Chromatography cellulose media
> Chromatography cellulose media
Whatman offers an extensive product line of chromatography cellulose media for applications ranging from the separation of biopolymers such as proteins, peptide and hormones to the purification of monoclonal antibodies, vaccines, synthetic pharmaceuticals and agrochemicals. 

Technological innovations in product development and manufacturing processes and a dedication to excellence are combined to ensure batch-to batch reproducibility of these chromatography cellulose media.
 
 

 Chromatography paper

Chromatography paper
>Chromatography paper
Whatman chromatography papers are the most widely used papers for chromatography worldwide. This acceptance and usage reflects the purity, high quality and consistency of Whatman papers. These qualities are relied upon by chromatographers and essential to successful reproducible chromatography. Whatman chromatography paper media are made from specially selected cotton cellulose. They are rigorously quality controlled for characteristics important to the chromatographer and to ensure uniformity within the grade. The chromatography paper product line includes standard cellulose and ion exchange grades. 
 
 

Bulk silica media for column chromatography

Bulk silica media for column chromatography
> Bulk silica media for column chromatography
The Whatman bulk silica media is used for low pressure columns. Same base silica and chemistries as those used for Whatman TLC plates. Particularly useful for scaling up separations optimized by TLC. In addition, Whatman offers an all-purpose media for general column chromatography. Applicable for preparative low pressure column separations scaled up from Partisil analytical HPLC columns.
 

Features and Benefits

  • High resolution
  • Good flow characteristics
  • High surface area
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for Low Pressure Column Chromatography
4771-010 LPS-2 low pressure silica gel 37–53 Adsorption 450 m2/g; may be dry packed 1 kg
4776-001 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 100 g
4776-005 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 500 g
 
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for General Column Chromatography
4132-100 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 100 g
4132-301 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 1 kg
4790-010 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 1 kg
4790-050 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 5 kg
4790-250 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 25 kg
4791-005 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 500 g
4791-010 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 1 kg
4791-050 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 5 kg
4791-250 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 25 kg

NEW Purasil 60Å Silica Gel

Silica Gel Media for Flash Chromatography 
Whatman Purasil high purity silica gel provides an excellent separation medium for flash chromatography purification of target molecules. Narrow particle size distribution and minimal fines enable fast separations with no loss of chromatographic performance.

Features and Benefits

  • High resolution
  • Excellent flow rates
  • Direct scalability
Typical Data
Iron Content <0.02%
Chloride Content <0.10%
Loss on Drying <7%
pH (10% suspension) 7 ± 0.5
Pore Volume 60–80 mL/g
Surface Area 500–600 m2/g
Ordering Information
Catalogue Number Product Dimensions
4745-010 230–400 mesh (38–63 µm) 1 kg
4745-250 230–400 mesh (38–63 µm) 25 kg
4746-010 70–230 mesh (63–210 µm) 1 kg
4746-250 70–230 mesh (63–210 µm) 25 kg
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Nucleic Acid

 

Collection, storage and purification
 
 
Collection, storage and purification
> Whatman FTA cards
FTA® Technology 
Collect, Transport, Archive and Isolate Nucleic Acids - All at Room Temperature!
FTA Cards utilize patented Whatman FTA Technology that simplifies the handling and processing of nucleic acids.
 

FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA Cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms. Try FTA, and you'll soon find it's an indispensable part of your DNA toolbox. US Patent Nos. 5496562, 5756126, 5807527, 5972386, 5985327 and other patents pending

Features and Benefits

Collection, storage and purification

Electron micrograph showing DNA entrapped within the FTA matrix (magnification x 10,000).

Capture nucleic acid in one easy step

  • Captured nucleic acid is ready for downstream applications in less than 30 minutes
  • Nucleic acids collected on FTA Cards are stable for years at room temperature
  • FTA Cards are stored at room temperature before and after sample application, reducing the need for laboratory freezers
  • Suitable for virtually any cell type
  • Indicating FTA Cards change colour upon sample application to facilitate handling of colourless samples
  • FTA Cards are available in a variety of configurations to meet application requirements
  • Custom configurations are available on request

pplications

  • Forensics
  • Transgenics
  • Transfusion Medicine
  • Plasmid Screening
  • Food & Agriculture Testing
  • Drug Discovery
  • Genomics
  • STR Analysis
  • Animal Identification
  • Diagnostics
  • Pharmacogenomics
  • Molecular Biology

Capture Nucleic Acids in One Easy Step

Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibres of the card. The nucleic acids remain immobilized and are stabilized for transport, immediate processing or long-term room temperature storage.

Since captured nucleic acids are stabilized, FTA Cards facilitate sample collection in remote locations and simplify sample transport. For example, you can collect samples deep in a rain forest without worrying about immediate refrigeration. Ship your samples back to the lab without expensive special handling or dry ice, and process at your convenience.

Indicating FTA Cards are recommended for colourless samples. These FTA Cards change from pink to white when sample is applied, verifying the location of the sample.

FTA Cards Used With Virtually Any Sample Type

  • Blood
  • Cultured Cells
  • Buccal Cells
  • Plant Material
  • Bacteria
  • Plasmids
  • Microorganisms
  • Solid Tissue
  • Viral Particles
  • M13 Plaques
  • ...and more

Captured Nucleic Acid is Ready for Downstream Applications in Less than 30 Minutes
Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification Reagent and rinse with TE-1 buffer. DNA on the washed punch is ready to use in applications such as PCR, RFLP analysis and RT-PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.

Store Nucleic Acids at Room Temperature for Years
Genomic DNA stored on FTA Cards at room temperature for over 14 years (and counting) has been successfully amplified by PCR. In contrast, genomic DNA stored at room temperature on non-FTA Cards for over six months did not amplify.

Sample integrity is optimized when FTA Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.

FTA Cards offer a compact room-temperature storage system that reduces the need for precious freezer space.

FTA Classic Card 
Four sample areas for application of up to 500 µL whole blood or 100 µL plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples on one card. Different samples can be processed independently.

Indicating FTA Classic Card 
Same as FTA Classic Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with colourless samples such as buccal or culture cells.

FTA Mini Card 
Two sample areas for application of up to 250 µL whole blood or 50 µL plant homogenate per card. Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.

Indicating FTA Mini Card 
Same as FTA Mini Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Micro Card 
One sample area for application of up to 125 µL whole blood or 25 µL plant homogenate per card. Recommended when only one sample is needed.

Indicating FTA Micro Card 
Same as FTA Micro Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Gene Card 
An FTA card enclosed in a rigid card frame. Three sample areas for application of up to 225 µL whole blood or 30 µL plant homogenate per card. Can be utilized in many automatic dispensing/pipetting systems when used with the FTA Gene Card Tray (WB100030).

PlantSaver™ FTA Card 
Plant friendly FTA Card, in a Classic Card format. Features a laminated flap that allows you to vigorously pound the plant sample into the FTA matrix without damaging the FTA Card.

FTA Kit 
Includes: 25 FTA Micro Cards, 2 vials purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

FTA Starter Pack 
Includes: 1 FTA Classic Card, 1 FTA Mini Card, 1 FTA Micro Card, 1 Indicating FTA Mini Card, 1 FTA Indicating Micro Card, 2 foam-tipped applicator swabs, 1 multi-barrier pouch and desiccant, 1 vial purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

Blood Stain Card 
Contains non-FTA absorbent filter paper for the collection and transport of blood and body fluids. Intended for short-term handling of specimens and for protein or metabolite studies. For long-term storage of nucleic acids, Whatman FTA Cards are recommended. There are four sample areas for application of up to 500 µL whole blood per card. Convenient for multiple applications of the same specimens or collection of multiple specimens on one card. Different samples can be processed independently.

Ordering Information - Whatman FTA ®Technology - Nucleic Acid Collection, Storage and Purification
Catalogue Number Description Cards/
Pack		 Sample Areas/
Card		 Maximum Volume/
Sample Area (µL)		 Maximum Total
Volume/Card (µL)
WB120067 FTA Kit 25 — — —
WB120061 FTA Starter Pack N/A — — —
WB120305 FTA Classic Card 25 4 125 500
WB120205 FTA Classic Card 100 4 125 500
WB120306 Indicating FTA Classic Card 25 4 125 500
WB120206 Indicating FTA Classic Card 100 4 125 500
WB120355 FTA Mini Card 25 2 125 250
WB120055 FTA Mini Card 100 2 125 250
WB120356 Indicating FTA Mini Card 25 2 125 250
WB120056 Indicating FTA Mini Card 100 2 125 250
WB120310 FTA Micro Card 25 1 125 125
WB120210 FTA Micro Card 100 1 125 125
WB120311 Indicating FTA Micro Card 25 1 125 125
WB120211 Indicating FTA Micro Card 100 1 125 125
WB120208 FTA Gene Card 25 3 75 225
WB120208 FTA Gene Card 100 3 75 225
WB120365 PlantSaver FTA Card 25 4 — —
WB120065 PlantSaver FTA Card 100 4 — —
WB100014 Blood Stain Card, non-FTA 100 4 125 500
WB120217 FTA Card/Pouch/Desicant 1000 — — —
WB120216 FTA Gene Card/Pouch/Desiccant 1000 — — —
 

For Collection, Storage, Processing an Shipping FTA Cards

FTA® reagent and accessories
FTA Purification Reagent
  • For purification of nucleic acids stored on FTA Cards
  • Ensures superior quality DNA for PCR or RFLP analysis
  • Removes heme, PCR inhibitors and other potential contaminants
  • Non-toxic, hypoallergenic aqueous solution

FTA Gene Card Tray

  • Holds 2 FTA Gene Cards for use in automatic dispensing/pipetting systems
  • Tray footprint conforms to SBS standards

 Harris Micro Punches

FTA® reagent and accessories
> Harris Micro Punches

Harris Micro Punches (1.2 mm or 2.0 mm) and Cutting Mat

  • Recommended for the precise punching of FTA Cards. No sample carryover when recommended procedures are used. Tips provide up to 2000 punches. Polished steel tip is case hardened and can be sterilized. The cutting mat ensures clean sample cuts and extends the life of the cutting tip
  • 1.2 mm punch recommended for use with FTA Cards containing whole blood and samples with high DNA content
  • 2.0 mm punch recommended for use with FTA Cards containing buccal cells, plasmids and other samples with lower DNA content

Sterile Foam Tipped Applicator

  • For the collection of saliva and buccal cells
  • Non-abrasive foam head is same size as sample area on Indicating FTA Cards to facilitate sample application

Sterile Omni Swab

This is a non-invasive device for the collection of saliva and cheek buccal cells. Sterile Omni Swab features a unique brush-like swab head that easily ejects from the stem of the swab for transfer of samples into tubes and multiwell plates. Sterile Omni Swabs are pre-sterilized and individually wrapped for single use.

 Multi-Barrier Pouches

FTA® reagent and accessories
> Multi-Barrier Pouches

Multi-Barrier Pouches

Large

  • For transporting or storing FTA Classic Cards
  • Seven laminated layers that protect the card from exposure to gas or liquid contamination
  • Tamper-evident seal maintains sample security
  • Outer paper surface for labelling or writing

Small

  • Same construction in a smaller size for storing FTA Gene Cards, Mini Cards or Micro Cards

FTA Card Mailer

  • For use with the Multi-Barrier Pouches to transport FTA Cards in accordance with US Postal Service requirements

Storage Desiccant Packets

  • Ensure that FTA Cards remain dry during transport or storage
  • Changes from blue to pink to indicate absorption of moisture
Ordering Information - FTA Reagent and Accessories
Catalogue Number Description Quantity/Case
WB120204 FTA Purification Reagent 500 mL
WB100030 FTA Gene Card Tray 20
WB100032 Sterile Foam Tipped Applicators 100
WB100005 Harris Micro Punch 1.2 mm (with Mat) 1
WB100006 Replacement Tip 1.2 mm 1
WB100007 Harris Micro Punch 2.0 mm (with Mat) 1
WB100008 Replacement Tip 2.0 mm 1
WB100020 Replacement Cutting Mat 1
WB100010 Multi-Barrier Pouch, Large (9 x 15 cm) 500
WB100011 Multi-Barrier Pouch, Small (8 x 7 cm) 500
WB100016 FTA Card Mailer 50
WB100003 Desiccant Packets (1 gm) 1000
WB100021 Bloodstain Card/Pouch/Desiccant 500
WB100025 Harris Micro Punch 1.2 mm replacement plunger 1
WB100026 Harris Micro Punch 2.0 mm replacement plunger 1
WB100028 Harris Uni-Core 1.2 mm punch 4
WB100029 Harris Uni-Core 2.0 mm punch 4
WB100035 Sterile Omni Swab 100
 
GenSpin™ Genomic DNA Purification Kit
 

GenSpin™ genomic and plant DNA purification kits> GenSpin genomic and plant DNA purification kit

GenSpin™ Genomic DNA Purification Kit 
The GenSpin Purification Kit is designed to purify high quality, PCR-ready, single stranded DNA in solution from whole blood and cultured cells in as little as 25 minutes.

This simple protocol uses a single micro centrifuge tube and small sample volume (5-50 µL) to produce high quality DNA for amplification by PCR. DNA for up to 80 amplification reactions can be obtained from a 50 µL fresh or anticoagulant-treated blood sample.

GenSpin incorporates patented FTA technology, which lyses cell and nuclear membranes on contact. The DNA is reversibly entrapped within this type of FTA filter matrix and can be stored for weeks at room temperature prior to purification. Cellular debris and proteins are removed by washing with GenSpin Buffer and TE-1 Buffer using a centrifuge. The purified DNA is released from this specialized filter matrix by heat elution and is ready for immediate PCR amplification.

Features and Benefits

  • Simple, single tube protocol. Purify single stranded DNA from whole blood and cultured cells in less than 25 minutes
  • FTA technology lyses cells, denatures proteins and inactivates viral contaminants. Allows room temperature storage of DNA and safe sample transport and handling prior to purification
  • High quality DNA. Enables full length PCR amplification
  • Highly efficient method. Enables purification of sufficient DNA for up to 80 amplification reactions from a 50 µL blood sample
  • No precipitation steps. DNA is ready for immediate analysis
GenSpin Genomic DNA Purification Kit Contents
Quantity Item
50 DNA Isolation Filter Baskets in 2 mL microcentrifuge tubes
50 2 mL microcentrifuge tubes
1 GenSpin Buffer 110 mL
1 Nuclease Free Water 25 mL
1 User Manual
Ordering Information - GenSpin Genomic DNA Purification Kits
Catalogue Number Description Size
WB120005 GenSpin DNA Purification Kit 50 purifications
WB120111 GenSpin Sample Kit 5 purifications
GenSpin Plant DNA Purification Kit Contents
Quantity Item
50 GenSpin Purification Tube with Filter Basket
50 GenSpin Collection Tube
1 bottle Homogenization Buffer 25 mL
1 bottle Wash Reagent 60 mL
1 bottle Rinse Reagent 60 mL
1 Instruction Booklet
Ordering Information - GenSpin Plant DNA Purification Kits
Catalogue Number Description Size
WB120046 GenSpin Plant Kit 50 Purifications
SWB120046 GenSpin Plant Sample Kit 5 Purifications
Table 1. Plant Species - Typical DNA Yields from 10 mg of Young Leaf Tissue
Plant Species Double Stranded DNA Yield (ng)
Alfalfa 800
Arabidopsis thaliana 110
Barley* 670
Brassica sp. 800
Corn* 120
Cotton 450
Potato 2200
Rice 120
Ryegrass 340
Soybean* 500
Spinach 340
Tobacco 1100
Tomato 1800
Wheat* 710

*Extraction of these plant species requires the addition of DTT to Homogenization Buffer. 
DNA yields can vary depending on plant species, tissue age and growing conditions. 
Double-stranded DNA was quantified using PicoGreen™ Reagent
Table 2. Comparison of GenSpin Plant and a Common Manual DNA Isolation Method
Extraction Time GenSpin Plant 25 minutes Manual Method* 90 minutes*
Homogenization Room Temperature Liquid Nitrogen
Precipitation/Resuspension Not Required Required
All Reagents Aqueous Yes No
Archiving Capability Yes No
PCR of Low-Copy Loci Yes Yes
Double-Stranded DNA Yes Yes
gDNA Isolation from Other Cell Types (bacteria, blood) Yes No
Pathogen Inactivation Yes No

* Manual method (Dellaporta et. al. 1983), does not include time required for full resuspension after DNA precipitation. 
Reference: Dellaporta et. al. (1983) A plant DNA Minipreparation. Plant Molecular Biology Reporter 1:19-21

GenSpin™ Plant DNA Purification Kit

The GenSpin Plant DNA Purification Kit is designed for the rapid preparation of double-stranded DNA in solution from small quantities of plant material for PCR analysis. Using a single microcentrifuge tube, this simple protocol enables the recovery of DNA for more than 50 amplification reactions from just 10 mg of plant tissue. The small sample capability is ideally suited for rapid analysis studies such as identification of genetically-modified plants and cultivar screening.

Plant material is homogenized at room temperature and applied to the GenSpin Filter Basket, which incorporates FTA technology to immediately stabilize the DNA at room temperature. Nucleases are inactivated and the DNA is protected from UV and environmental damage. The immobilized DNA is entrapped in the fibres of the matrix and can either be purified immediately or stored at room temperature for more than 4 weeks. The filter is washed with two reagents to remove contaminants that would inhibit PCR. The DNA is then eluted from the filter by heating and collected by centrifugation.

Features and Benefits

  • Simple, single tube protocol. Eliminates need for organic solvents, liquid nitrogen and time-consuming precipitation steps
  • Fast purification of DNA. Purify DNA in less than 30 minutes for quick sample screening. Up to 50 amplifications from only 10 mg of plant material
  • PCR-ready double-stranded DNA. Reliable amplification of DNA for a wide range of applications including cultivar screening and ID of genetically modified plants
  • FTA technology protects DNA from degradation. Enables room temperature storage for weeks
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Nucleic Acid Sample Preparation

FTA classic card
 
Nucleic acid sample preparation
> FTA classic card
Whatman has been manufacturing quality paper products since 1740 and is recognised as the world leader in filter separation technologies and products. 

With all this knowledge and remarkable testimonies to quality, Whatman has taken its products to a new level, in the genomics/proteomics industry. Whatman's area of expertise in this evolving industry lies in sample preparation, where our FTA® Cards - a revolutionary patented technology for collecting, transporting, purifying and archiving DNA and RNA all from a single card stored at room temperature - have become market leaders.

The nucleic acid sample preparation products incorporate unique Whatman technologies, which offer several outstanding advantages to molecular biologists. These include the encapsulation of solid media into devices, DNA separation products, services and products designed for the collection, transportation, purification and analysis of nucleic acids. All of these new Whatman products create breakthrough applications that yield accurate results much faster than previously possible. Offering an extensive, leading-edge product range and an efficient contract service means that all your DNA processing requirements are met by one established provider.

Whatman offers an extensive range of products to facilitate genomic studies of humans, animals, plants and microorganisms. Collection, storage and analysis of DNA and RNA all benefit from the use of FTA and other Whatman tools.
 

3MM Chr and 17 Chr Blotting Papers

Blotting papers
3MM Chr and 17 Chr Blotting Papers

3MM Chr Whatman 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for general chromatography and electrophoresis. Flow rate is 130 mm/ 30 min.

17 Chr 
A thick (0.92 mm) and highly absorbent paper with a very high flow rate of 190 mm/ 30 min. Suitable for the heaviest loadings and ideal for preparative paper chromatography and electrophoresis.

Features and Benefits

  • Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
  • Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
  • Whatman 3MM Chr is considered the industry standard for blotting procedures
  • Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations
Ordering Information - Blotting Paper for Ethanol Cleanup
Catalogue Number Quantity/Case
96166729 25
Ordering Information - 3MM Chr Pure Cellulose Grade Sheets
Catalogue Number Size (cm) Quantity/Packs
3030-6185 11 x 14 100
3030-6132 12 x 14 100
3030-153 15 x 17.5 100
3030-6188 15 x 20 100
3030-221 18 x 34 100
3030-861 20 x 20 100
3030-6461 26 x 41 100
3030-347 35 x 43 100
3030-392 35 x 45 100
3030-335 31.5 x 35.5 100
3030-917 46 x 57 100
3030-931 58 x 68 100
3030-6189 4" x 5.25" 100
3030-6187 6" x 8" 100
3030-6185 11 x 14 100
17 Chr Pure Cellulose Grade Sheets
3017-915 46 x 57 25
 

  • Clone Archiving

CloneSaver™ Card

Clone archiving
> CloneSaver Card
FTA® Technology in 96 Well Format for High Throughput Applications

Designed for the collection, long-term storage and purification of plasmid and BAC DNA from bacterial clones in a 96 well format.

Prepare BAC and Plasmid DNA with Amazing Ease

  • Apply 5 µL bacterial culture, re-suspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing
  • Bacteriophages are inactivated
  • DNA is easily accessible for downstream applications
  • Store up to 96 samples on each card

Store Sample DNA for Years at Room Temperature 
Plasmid DNA stored on CloneSaver Cards is stable at room temperature for at least 4 years...and counting.

DNA is Easily Accessible for Downstream Applications

Transformation 
Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods.

PCR 
Immobilized plasmid DNA on a CloneSaver Card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies.

Sequencing 
Plasmid DNA eluted from a CloneSaver punch can be amplified by rolling circle amplification, such as Amersham Bioscience's TempliPhi™ and then sequenced without the need for culture re-growth and plasmid purification.

Clone archiving
> CloneSaver Card with Multi-Barrier Pouches

CloneSaver™ Resealable Multi-Barrier Pouches

Used for transporting or storing the CloneSaver Card. The pouch is constructed with seven laminated layers that protects the card from exposure to gas or liquid contamination. There is a zip-lock resealable closure for easy access to the CloneSaver Card. The tamper-evident seal maintains sample security and the outer paper surface can be used for labelling or writing.

SPOT CloneSaver/BloodSaver Holder

SPOT is a rigid frame that allows automated spotting to standard CloneSaver and BloodSaver cards. It keeps the card flat for uniform and precise spotting of your biological samples. A 96 well card is easily inserted into the SBS-compatible frame, which can then be placed onto a liquid-handling deck just like a multiwell plate.

Clone archiving
> SPOT Holder
The SPOT holder is compatible with standard liquid handlers manufactured by companies such as Beckman Coulter and Tecan Instruments.

CloneSaver Starter Kit

Includes: 2 CloneSaver Cards, 2 Uni-Core Punches (2 mm) with cutting mat and instructions.

Ordering Information - Clone Archiving
Catalogue Number Description Cards/Pack Sample Areas/Card Maximum Volume/Sample Area (µL) Maximum Total Volume/Card (µL)
WB120052 CloneSaver Starter Kit N/A N/A N/A N/A
WB120028 CloneSaver Card 5 96 well format 5 480
WB100024 CloneSaver Resealable Multi-Barrier Pouch 50 N/A N/A N/A
WB100034 SPOT Holder for Semi-Automated Spotting 1 N/A N/A N/A
 
 

EasyClone 384™ PlateNucleic acid sample preparation

Clone archiving
> EasyClone 384 Plate
EasyClone 384™ Plate
The Whatman EasyClone 384 replaces traditional freezer storage methods and offers first of its kind, single device for entire sample archiving and purification workflow, enabling faster DNA mining and discovery. 

The EasyClone 384 plate can be used by biotechnology, pharmaceutical, government and academic laboratories for the archiving, shipping and purifying of clones - all at room temperature.

Relying on proven Whatman FTA® technology, which allows for the collection, storage and purification of DNA from a variety of biological samples, EasyClone consists of a 384-well storage and extraction plate with a piercable foil bottom and FTA disks pre-cut into each well.  The design and format of the EasyClone 384 plate enables the genomics market to use FTA as a replacement for both ultra-low archiving and purification kits.

Ordering Information - EasyClone 384™ Plate
Catalogue Number Description Quantity/Case
WB120069 EasyClone 384 plates 50
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Speciality Microplates

Clear view microplates

Speciality microplates
> Clear view microplates
Clear view microplates
Whatman Clear View microplates have optically clear polymer bottoms. They eliminate the need for numerous transfer steps by providing the means to grow, observe, count and assay cells in a single device. Tissue culture treatment facilitates cell adhesion. Whatman Clear View microplates have a very low visible-wavelength absorbance background.
 
Ordering Information - Clear View Microplates
Catalogue Number Well Format Well Volume (µL) Plate Material Quantity/Case
No Surface Treatment, No Lid
7706-2380 96 300 Black Polystyrene 50
7706-2103 384 100 Black Polystyrene 50
Tissue Culture Treated, Irradiated with Lid
7716-2380 96 300 Black Polystyrene 50
7716-3380 96 300 White Polystyrene 50
 
 
 

Glass bottom microplates

Speciality microplates
> Glass bottom microplates
Glass bottom microplates
Whatman Glass Bottom microplates are designed for high-sensitivity detection including fluorescent and luminescent detection and scintillation counting, where extremely low backgrounds with no crosstalk are needed. Glass Bottom microplates are designed to provide optically clear as well as optically flat surfaces. This ensures confluence and planarity for confocal imaging and detection techniques. 

They are suitable for FRET and GFP. The glass bottom plate allows the bottom of the plate to be positioned easily for microscope objectives. This is the plate of choice for the Zeiss Confocal Microscopes. The glass bottom microplates are available in clear and black in a 96 well format.

Features and Benefits

  • Superior Optical Clarity
  • Optics using single or dual wavelength probe
  • Sensitive
  • Absolute flatness

Applications

  • Receptor-Ligand Interaction
  • DNA-Protein Interaction
  • Enzyme Studies
  • Cell based assays
Ordering Information - Glass Bottom Microplates
Catalogue Number Well Format Well Volume (µL) Plate Material Characteristics Quantity/Case
Tissue Culture Treated, Irradiated with Lid, Standard Skirt
7716-2375 96 300 Black Polystyrene Glass 5
Tissue Culture Treated, Irradiated with Lid, Skirtless for Microscopy
7716-2370 96 300 Black Polystyrene Glass 5
No Surface Treatment, Standard Skirt
7706-2375 96 300 Black Polystyrene Glass 5
No Surface Treatment, Skirtless for Microscopy
7706-1365 96 300 Clear Polystyrene Glass 5
7706-2370 96 300 Black Polystyrene Glass 5
 
 
 

UniCell™ microplates

Speciality microplates
> UniCell microplates
UniCell™ microplates
The UniCell 24 microplate is a versatile product that is specifically designed for cell culture. The UniCell 24 consists of three components: 
  • 24 well filtration microplate containing a polycarbonate membrane with a pore size 0.4 µm
  • 24 well feeder tray with round wells which have a volume of 3.5 mL
  • Polystyrene lid cover

The polycarbonate membrane is ideal for cell culture because it is not toxic to cells and will not inhibit cell growth. It is the ideal material to allow formation of a confluent monolayer of mammalian cells.

The membrane becomes translucent when wet and retains its strength, allowing for the harvesting of cells either by sloughing or by mechanical removal off the membrane. The growth well, contained in the top microplate, sits neatly inside the feeder tray. Each well is completely sealed and sits in its own individual feeder well. The complete UniCell 24 is supplied irradiated and tissue culture treated. The clearance between the bottom of the membrane and the bottom of the feeder tray is 2mm.

Applications

  • Permeability studies
  • Co-cultivation
  • Tissue resistance
  • Cell migration
  • Toxicology
Ordering Information - UniCell™
Catalogue Number Well Format Plate Material Filter Media TC Treated/Irradiated Quantity/Case
7703-1400 24 Polystyrene 0.4 µm polycarbonate membrane Yes 5
 
 
 

UniPCR™ microplates

Speciality microplates
> UniPCR microplate
UniPCR™ microplates
The UniPCR* line of microplates are designed to meet the demanding needs of high throughput genomic laboratories and are compatible with most thermocyclers. Suitable for PCR amplification, these microplates are produced with a special polymer for good thermal conductivity.
*PCR is patented by Hoffman-La-Roche Ltd
Ordering Information - UniPCR™ Microplates
Catalogue Number Well Format Well Volume (µL) Plate Material Characteristics Quantity/Case
7703-1901 96 200 Thin Walled, Clear Copolymer PCR thermal cyclers 50
7703-1305 384 25 Thin Walled, Clear Copolymer PCR thermal cyclers 50
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Speciality Products

Benchkote

Benchkote® and Benchkote Plus™
Benchkote 
Benchkote is an absorbent, impermeable material designed to protect laboratory surfaces against hazardous spills. The material features a high quality, smooth, absorbent Whatman paper which quickly absorbs liquid spills and a laminated polyethylene layer that prevents flow through to the working surface. After use the sheet is incinerated or disposed of according to local regulations. This makes both Benchkote and Benchkote Plus excellent products that comply with OSHA Regulation 29CFR 1910.1030 for Occupational Exposure to Bloodborne Pathogens. 

Benchkote Plus 
Benchkote Plus is a thicker, more absorbent material for more demanding applications and can absorb in excess of 0.75 litres of water per square meter.

Features and Benefits

  • Material is very strong, making it tear resistant, wet or dry
  • Smooth white surface can be written on with ink or pencil and lies flat
  • Suitable for saturation with disinfectant to protect benches where pathogens and other bacteria are present
  • Use polyethylene side up to collect deposits without absorption
  • Paper side quickly absorbs liquids preventing liquids from going through to the working surface
  • Spillages are trapped in the absorbent paper
  • Benchkote can be picked up and burnt very easily after use; the polyethylene layer does not melt or drip but is rapidly consumed in the flames

Applications

  • Containing radiochemical spillage and avoiding contamination
  • Recovering spillage of expensive materials
  • Protecting hard surfaces to lessen impact
  • Water or solvent wick for humidity chambers
  • Lining of chemical cabinets, laboratory bench drawers and laboratory hoods
Ordering Information
Catalogue Number Description Dimensions Quantity/Pack
Benchkote
2300-916 Sheets 46 cm x 57 cm 50
2300-594 Pads 46 cm x 57 cm 50
2300-731 Reel 46 cm x 50 m 1
2300-772 Reel 92 cm x 50 m 1
Benchkote Plus
2301-6150 Sheets 50 cm x 60 cm 50
2301-6160 Reel 60 cm x 50 m 1
 
 
 

CryptTest™ membrane filter cartridge

CryptTest™ membrane filter cartridge
> CrypTest membrane filter cartridge
Whatman introduces a new, convenient membrane filter cartridge for the concentration and recovery of water-borne protozoan cysts and oocysts which has been approved by the EPA for Method 1623, Cryptosporidium and Giardia in Water by Filtration. 

The CrypTest Cartridge captures protozoan cysts and oocysts from sample water pumped through the disposable cartridge contained in a re-usable housing. The CrypTest contains a 1.0µm pore size Nuclepore® polycarbonate track etch membrane.

Protozoan cysts and oocysts are recovered from the membrane cartridge by using a simple backwashing procedure. The eluant, containing cysts and oocysts, is decanted from the housing into a collection vessel.

Further concentration, purification and separation by centrifugation, immunomagnetic separation or flow cytometry/cell sorting is then performed. Enumeration and confirmation of the cysts and oocysts is done using epifluorescence and DIC procedures.

Features and Benefits

  • Nuclepore polycarbonate track etch membrane provides total surface capture
  • Simple backwashing procedure yields consistently high recovery
  • Convenient for field sampling-sample water pumped through disposable cartridge contained in durable, reusable housing
  • Simple to use
Method
The CrypTest Cartridge was developed for efficient concentration and recovery of protozoan cysts and oocysts in water. The following method is recommended for processing water samples.

Materials and Equipment

  • CrypTest Cartridge - Catalogue Number 610064
  • Cartridge housing, AMETEK 5" clear polycarbonate - Catalogue Number 71503
  • 1/2" male pipe threads with male hose connectors (2)
  • Laboratory tubing, Tygon® formula R-3603, or equivalent, to which cysts/oocysts will not adhere
  • Flow control valve - 2 litres/minute (0.5 gallons/minute)
  • Water flow meter
  • Peristaltic pump
  • Ultrasonic bath

Elution Buffer

  • NaCl - 8.0g
  • KH2PO4 - 0.2g
  • Na2HPO4 (12H2O) - 2.9g
  • KCl - 0.2g
  • Sodium lauryl sulphate (SDS) - 0.2g
  • Tween 80 - 0.2ml
  • Antifoam A* - 0.02ml
  • Adjust volume to 1 litre with reagent water.
  • Adjust pH to 7.4 with 1 N NaOH or HCl.

*Sigma Chemical Co - Catalogue Number A5758.

CryptTest™ membrane filter cartridge
> Sampling from Carboy

Sample Filtration

Key
A - Sample
B - Filter Inlet
C - Filter Outlet
D - Peristaltic Pump
E - Flowmeter
F - To Waste

1. Clean and rinse cartridge housing 
2. Attach male pipe threads to inlet and outlet of cartridge housing cap. 
3. Attach new or well-cleaned tubing to male hose connectors on inlet and outlet of cartridge housing. Secure both with band clamps. 
4. Install the cartridge filter in the housing. 
5. When filtering sample water from a carboy, thread the outlet tubing through the peristaltic pump and immerse the open end of the inlet tubing in the carboy. Alternatively attach open end of inlet tubing directly to feed line. 
6 Turn on pump and adjust the flow rate to 2 litres/min. 
7 Turn off the pump when the desired sample volume has been filtered.

CryptTest™ membrane filter cartridge
> Sampling from Feed Line

Key
A - Sample Tap
B - Pressure Regulator & Gauge
C - Filter in Housing
D - Flow Totalizer
E - Flow Control Valve
F - Drain Hose

Backwashing the Cartridge to Elute Cysts/Oocysts

1 Detach tubing from housing inlet and decant any water remaining in the inlet side of the housing into a 1 litre sample bottle. 
2 Attach the housing outlet to the discharge tubing of the peristaltic pump. Place the free end of this tubing into a beaker of elution buffer. 
3 Start the pump and run at approximately 400ml/min until the filter outlet core and the housing inlet cavity are filled (approximately 300ml). 
4 Cap the housing inlet and outlet and place the housing in the ultrasonic bath for 2 minutes. 
5 Remove the inlet cap and decant the liquid from the inlet side into the collection bottle. 
6 Repeat steps 3, 4 and 5 and then proceed to step 7. 
7 Connect housing outlet to regulated compressed air source and gradually increase pressure to 5 to 10psi as necessary to drive remaining elution buffer from the outlet side to the inlet side and into the collection bottle. 
After backwashing the cartridge and collecting the eluate, further purification, detection and enumeration of cysts/oocysts may be performed using EPA recommended methods.<

CryptTest™ membrane filter cartridge
> Backwash

Key
A - Eluting Solution
B - Peristaltic Pump
C - Filter Outlet
D - Filter Inlet

Cleaning the Cartridge Housing

The following procedure is recommended for cleaning the cartridge housing between samples.

1. Wash thoroughly with water and laboratory detergent, using a brush to clean all crevices. 
2. Soak in 6% sodium hypochlorite solution for 30 minutes. 
3. Rinse thoroughly with tap water. 
4. Rinse with laboratory water. 
 
 
 

pH Indicators and test papers

pH Indicators and test papers
Whatman offers a range of pH indicator and test papers to meet your specific needs. Made with traditional Whatman quality, these products combine ease-of-use with unsurpassed accuracy and consistency. 

The convenience of using indicator papers for the rapid determination of pH values has led to many applications in laboratories and industry.

Features and Benefits

  • Instant pH readings
  • Accurate for a wide range of routine pH testing
  • Inexpensive
  • Convenient and portable for field use

pH Indicators

Strips - Type CF 
Individual plastic support strips carry four different segments of dye-impregnated indicator papers. The resulting combination of colour differences gives an extremely clear and accurate visual pH value. All the dyes are chemically bonded to the paper and cannot be leached into solution; problems associated with contamination of the sample and resultant anomalous readings are avoided.

Strips Type CS 
Each test strip has a central segment of indicator dye and, printed alongside, 8 or more different colour segments marked with corresponding pH values for matching purposes. The pH test value can be read off by direct comparison of the test strip colour and the colour bars. Ideal for coloured solutions, when any changes in colour of the paper stock are automatically cancelled out.

Dispensers Type TC 
The strip has 3 separate indicator dye colour bands. The unique combination of colour change resulting from each test is compared with the colour-coded comparison chart, printed on the dispenser, giving improved speed and accuracy in reading.

Dispensers Type SR 
A full range and some narrow ranges in this popular pH indicator dispenser.

Indicator Books 
The book format is particularly suitable for educational and industrial use. In schools they are economical because the amount of paper per student can be carefully controlled.

Acid-Alkali Test Papers

Litmus Blue and Litmus Red 
These easy-to-use test papers facilitate a general test for acid or alkaline reaction. The change occurs around pH 5-8. They are particularly recommended for educational use.

Congo Red 
This test paper changes colour from blue to red in the range pH 3-5 for the determination of neutralization point in strong acid/weak alkali reactions.

Phenolphthalein 
This white paper changes to pink at pH 8.3 and becomes red at pH 10. It is useful for the determination of the neutralization point in weak acid/strong alkali reactions.

Specialized Test Papers

Lead Acetate Test Paper 
Used for detecting hydrogen sulphide, this rapid qualitative test paper when wetted with distilled water can detect as little as 5 ppm of H2S in the atmosphere or in a gas stream. Hydrogen peroxide can be detected with this paper by pre-blackening the paper in H2S. Concentrations as low as 4 ppm can be detected.

Potassium Iodide Test Paper 
Used for detecting chlorine and other oxidizing agents. In acid solution, oxidizing agents react with the iodide in the test paper to liberate iodine. The paper will turn blue in the presence of an oxidizing agent (e.g., CI2, Br2, H2O2, HNO2, etc.).

Ordering Information
Catalogue Number Type Description pH Range pH Unit Graduation Dimensions Packaging
pH Indicator Papers
2613-991 CF Color Bonded 0–14 1 6 mm x 80 mm Pack of 100 Strips
2614-991 CF Color Bonded 4.5–10.0 0.5 6 mm x 80 mm Pack of 100 Strips
2612-990 CS Integral Comparison Strip 1.0–12.0 1 11 mm x 100 mm Pack of 200 Strips
2626-990 CS Integral Comparison Strip 1.8–3.8 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2627-990 CS Integral Comparison Strip 3.8–5.5 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2628-990 CS Integral Comparison Strip 5.2–6.8 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2629-990 CS Integral Comparison Strip 6.0–8.1 0.3 11 mm x 100 mm Pack of 200 Strips
2630-990 CS Integral Comparison Strip 8.0–9.7 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2631-990 CS Integral Comparison Strip 9.5–12.0 0.5 11 mm x 100 mm Pack of 200 Strips
Dispensers
2611-628 TC Three Colors 1.0–11.0 1 10 mm x 5 m Pack of 1 Dispenser
2600-100A SR Standard Full Range 1.0–14.0 1.0–2.0 7 mm x 5 m Pack of 1 Dispenser
2600-101A SR Standard Narrow Range 0.5–5.5 0.5–1.0 7 mm x 5 m Pack of 1 Dispenser
2600-102A SR Standard Narrow Range 4.0–7.0 0.5 7 mm x 5 m Pack of 1 Dispenser
2600-103A SR Standard Narrow Range 6.4–8.0 0.5 7 mm x 5 m Pack of 1 Dispenser
2600-104A SR Standard Narrow Range 8.0–10.0 0.5 7 mm x 5 m Pack of 1 Dispenser

Speciality products
Ordering Information
Catalogue Number Description Packaging
Acid Alkali Test Papers
Dispensers
     
2600-201A Litmus Blue Pack of 1 Dispenser
2600-204A Phenolphthalein  
Books
     
2600-601 Lithmus Blue Carton of 10 packs of 10 Books
2600-602 Litmus Red 20 Leaves per Book
2600-500 pH Indicator Booklet 
pH 1 - 11		 10 Strips per Book
10 Books per Pack
10 Packs per Carton
Specialized Test Papers Dispensers
     
2602-500A Potassium Iodide 7 mm x 5 m

Universal Indicator papers

Universal indicator papers have been impregnated with a mixture of several indicators. On contact with the sample solution they assume a particular colour. A check against the colour comparison table supplied allows the pH to be determined.

Ordering Information - PANPEHA – universal indicator papers
Catalogue Number Measuring pH range pH color change Size (mm) Qty/Pkg
PANPEHA
10 362 030 1-11 1 7 mm x 5 m 1
PANPEHA Plus*
10 362 000 0-14 1 6 x 85 100
10 362 010 2-9 0.5 6 x 85 100
PANPEHA Nr. 112
10 360 005 0-14 0.5/1** 10 x 75 200
Litmus blue
10 360 300 8-5 blue-red 10 x 75 100
Litmus red
10 360 400 5-8 red-blue 10 x 75 100
Phenolphthalein
10 360 700 8.3-10 white-red 10 x 75 100

* non-bleeding
** from pH 0-9 in 0.5 pH steps; from pH 9-14 in 1 pH steps

Clinical Papers

Antibiotic assay disks

For determining the type of causal agent of infectious diseases and for checking their sensitivity to antibiotics and chemotherapeutic agents in vitro by means of the inhibition zone determination method. The antibiogram allows rational and selective chemotherapy. The test disks are coated with chemotherapeutic agents, placed on the inoculated nutrient agar and incubated. The size of the inhibition zone is a measure for the effectiveness of the substances.

Papers for clinical applications

  • Grade 557: Tallquist hemoglobin scale (for direct rapid testing of the hemoglobin value in blood).
  • Grade 470: Soft surface. For gelatinous samples. Used for the absorption of culture media, as blotting paper, for electrophoresis and amino acid chromatography.
Ordering Information - Antibiotic assay paper
Catalogue Number Size (mm) Qty / Pkg
Grade 557
10 320 390 95 x 135 1 booklet (250 leaves)
Grade 470
10 318 487 200 x 200 100
10 318 493 460 x 570 100
10 318 489 203 x 305** 25

* corresponds 4 x 51/4 inches
** corresponds 8 x  12 inches
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Diagnostic components

 

Having the expertise and manufacturing capability to customise fibrous products to meet very specific requirements, Whatman has an established reputation for supplying key components for a wide range of applications.

For today's diagnostic device manufacturers, time-to-market is more critical than ever. They seek cost-efficient business partnerships that yield consistent product performance and quality - at increasingly aggressive rates of development. In essence, they require experienced single-source suppliers with extensive product lines that address the full spectrum of diagnostic applications. That is why so many diagnostic companies partner with Whatman.

We offer a comprehensive family of high-quality, solid-phase media - from sample wicks, blood separators and conjugate release pads to absorbent sinks, sample collection/transport materials and dipsticks.

In addition, our in-depth line of nitrocellulose membranes and our proprietary FUSION (single layer matrix) technology provide proven performance for lateral flow assays.

What's more, our pioneering work in emerging applications - including track-etched membrane and molecular diagnostic technologies - helps companies remain on the forefront of breakthrough diagnostic solutions.

By combining the highest quality standardized solutions with unsurpassed technical expertise and support, we enable diagnostic companies to optimize their product offerings quickly and cost-effectively. We thereby accelerate a company's time-to-market and maximize their competitive advantage.
  • Diagnostic membranes
 
Diagnostic membranes
> Immunopore diagnostic membrane
 Immunopore ™ diagnostic membranes
Whatman Immunopore was the first nitrocellulose membrane specifically designed for lateral flow diagnostics in 1995. Since then, the manufacturing process has been continuously refined to increase consistency and expand the range of wicking rates. Whatman Immunopore products include a proprietary polymer in the membrane matrix to ensure rapid rewetting and low background signal-eliminating protein-binding interference commonly experienced with surfactants. 

Features and Benefits
 

  • High sensitivity. 
    Capture line shows high intensity, making low positives easy to read and improving test range.
  • High consistency. 
    Demonstrate outstanding within- and between-batch consistency for lateral wicking rates (see graphs), making test development more predictable.
  • Long shelf life. 
    No significant performance change after three years' storage under ambient conditions, simplifying membrane handling and storage operations.
  • Product versatility. 
    Strong results regardless of capture reagent or analyte type. Can be utilized in virtually all tests.

Product Range and Properties 
Immunopore SP 
Mylar cast membrane construction. Maximum sensitivity coupled with low flow/wicking rate. Ideal for use with low-viscosity samples when maximum capture line intensity is required.

Immunopore FP 
Mylar cast membrane construction. Excellent general membrane offers high capture line intensity coupled with faster flow/wicking rate.

Immunopore RP 
Mylar cast membrane construction. Fastest flowing membrane, yielding shorter test times while still retaining excellent capture line intensity and reproducibility.

Standard Sizes 
Available in 50m rolls of 25mm width, for other sizes please inquire.

Given the increasing demand for whole blood assays, Whatman offers a family of blood separators specifically designed to meet the strict requirements of the rapid diagnostics market.

Features and Benefits

  • Faster development. 
    The four distinct separator grades facilitate faster and simpler development of any whole blood assay.
  • Optimal performance. 
    All separators are optimized to consistently provide required separation rates with high serum yields and no red cell haemolysis.
  • Rapid separation. 
    Blood separation is completed in 30 to 120 seconds.

Product Range and Properties

Since product selection for specific blood separation applications can be complex, please consult the "Where to Start" chart for typical applications.

LF1 
Designed for use in lateral flow assays. Performs well with one or two drops of whole blood.

MF1 
Appropriate for lateral or vertical flow assays. Typically used for whole blood volumes between 30µL and 100µL.

VF1 
Suitable for vertical flow assays. Typically used in a multi-layer format for separating larger volumes of blood.

VF2 
Primarily a vertical separator that can be used in a single- or multi- layer format to separate a wide range of blood volumes.

Standard Sizes 
Reels are available in the following standard sizes:

12mm x 50m 
17mm x 50m

Blood Separation
Lateral Flow   Vertical Flow  
  Single Layer Multiple Layer
<30µL >30µL <100µL >100µL  
LF1 MF1     VF2 VF1  
20 20 Minimum volume of whole blood that can be used (µL)   40 40  
0.59 0.77 Minimum area of separator to separate the minimum volume (cm2)   0.47 0.52  
21.2 17.6 Typical volume of serum recovered from 40µL of whole blood   14.4 16.1  
86 73 Percentage of available serum obtained (%)   60 67  
46 57 Basis weight (g/m2)   125 121  
247 367 Thickness (µm@53kPa)   785 632
 
 
 
Conjugate release
> Conjugate release
Conjugate release materials are critical to lateral flow assays. To ensure strong assay performance, the conjugate must dry without damage or aggregation and release rapidly when the sample is applied. For consistent, reliable results involving conjugate release, diagnostic manufacturers rely on Whatman products. 

Features and Benefits

  • Choice of product. 
    Rapid Release materials are optimized to provide improved performance without blocking, while Standard Release materials allow the development of proprietary blocking protocols.
  • Inherently hydrophilic conjugate pads. 
    Do not require treatment prior to conjugate application, decreasing time required for diagnostic kit production.
  • Innovative design.
    Enables the conjugate to dry as a solid phase without colloidal system deterioration. Open structure allows rapid pad penetration by both conjugate and sample.
  • Product versatility. 
    Can be used as combined sample wick/conjugate release pads when additional wicks are not desired.

Product Range and Properties

Rapid 24/Rapid 27 
No additional blocking step is required, accelerating assay development and simplifying manufacturing. Typically release more conjugate than untreated pads, reducing reagent costs. Pads rewet rapidly and capture line intensity is increased, significantly improving performance. Rapid 24 has higher absorption capacity and greater flexibility, while Rapid 27 has greater tensile strength.

Standard 14/Standard 17 Untreated grades ideal for conjugate pad optimization for particularly sensitive assays. Manufactured to enable optimization giving the same results every time. Pads rewet quickly and naturally without requiring surfactant treatment, facilitating simplified production. Standard 14 has higher absorption capacity and greater flexibility, while Standard 17 has greater tensile strength for easier manufacturing.

Standard Sizes 
Reels are available in the following standard sizes:

12mm x 50m 
17mm x 50m 
22mm x 50m 
27mm x 50m

Typical Test Data - Conjugate Release Materials
Grade Thickness (µm@53kPa) Absorption Capacity (mg/cm2) Max. Pore Size (µm) % Release of Gold Conjugate (after 90 sec)
Rapid 24 340 55 22 89
Rapid 27 365 40 22 86
Standard 14 355 55 23 75
Standard 17 370 35 23 75
 
 
 

Track etched membranes

Track etched membranes
> Track etched membranes
Whatman is actively involved in state-of-the-art R&D initiatives designed to keep our customers at the forefront of diagnostic technology. We provide our vision for the field's most important emerging applications and our assessment of the critical role Whatman products will play as those applications help our partners achieve competitive advantage. 

Track etched membranes (TEMs) are extremely thin yet highly controlled membranes. Traditionally, TEMs have been used for sample filtration before critical applications such as HPLC.

Broad Applicability Across Applications

The properties that make TEMs ideal for applications like filtration are becoming increasingly important for diagnostic tests.

Cell capture. 
Tightly controlled filtration properties allow you to capture cells of a specific pore size on the membrane surface. Enables cells to be detected in a clearer environment, yielding improved accuracy.

Particle capture and latex agglutination. 
The distinct pore size of TEMs enables the capture of particles of known sizes. Now you can construct assays in which capture reagents are bound to a latex bead (i.e., bead capture assays) or the antigen causes particle agglutination whereby the agglutinated clusters are captured on the membrane surface.

Biosensors. 
With biosensors, it is important to control liquid migration rates onto the sensor and prevent potential contaminants from reaching the sensor surface. The flow properties of a TEM can be chosen to yield the correct flow of liquids or gases through the membrane in order to obtain optimal sensitivity. It is also possible to modify surface properties so that membranes will serve as barriers to contaminants.

Erythrocyte deformability testing. 
Since healthy erythrocytes are highly flexible, they will readily change shape to pass through small openings. In several haemopathies, the ability of the cell to flex is reduced to the extent that it can no longer pass through a small aperture. When TEMs are used as a barrier to abnormal erythrocytes, it is possible to test for disease states.

In short, the degree of control that is possible in TEM manufacturing not only gives rise to a number of applications in which standard materials would fail, but also makes process validation and regulatory control easier and more intuitive.

Features and Benefits

  • Defined pore size. 
    The claimed nominal pore size is the largest pore size present in the membrane. The intra-lot variation in pore size is typically between two and three percent.
  • Biologically inert. 
    Since TEMs are manufactured from PC, the possibilities of assay interference are remote.
  • Control of flow rate. 
    TEMs are available with hydrophobic or hydrophilic surfaces in a range of pore sizes and pore densities. The flow rate of liquid through the membrane can therefore be tightly controlled.
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Whatman Clone archiving

  • Clone Archiving

CloneSaver™ Card

Clone archiving
> CloneSaver Card
FTA® Technology in 96 Well Format for High Throughput Applications

Designed for the collection, long-term storage and purification of plasmid and BAC DNA from bacterial clones in a 96 well format.

Prepare BAC and Plasmid DNA with Amazing Ease

  • Apply 5 µL bacterial culture, re-suspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing
  • Bacteriophages are inactivated
  • DNA is easily accessible for downstream applications
  • Store up to 96 samples on each card

Store Sample DNA for Years at Room Temperature 
Plasmid DNA stored on CloneSaver Cards is stable at room temperature for at least 4 years...and counting.

DNA is Easily Accessible for Downstream Applications

Transformation 
Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods.

PCR 
Immobilized plasmid DNA on a CloneSaver Card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies.

Sequencing 
Plasmid DNA eluted from a CloneSaver punch can be amplified by rolling circle amplification, such as Amersham Bioscience's TempliPhi™ and then sequenced without the need for culture re-growth and plasmid purification.

Clone archiving
> CloneSaver Card with Multi-Barrier Pouches

CloneSaver™ Resealable Multi-Barrier Pouches

Used for transporting or storing the CloneSaver Card. The pouch is constructed with seven laminated layers that protects the card from exposure to gas or liquid contamination. There is a zip-lock resealable closure for easy access to the CloneSaver Card. The tamper-evident seal maintains sample security and the outer paper surface can be used for labelling or writing.

SPOT CloneSaver/BloodSaver Holder

SPOT is a rigid frame that allows automated spotting to standard CloneSaver and BloodSaver cards. It keeps the card flat for uniform and precise spotting of your biological samples. A 96 well card is easily inserted into the SBS-compatible frame, which can then be placed onto a liquid-handling deck just like a multiwell plate.

Clone archiving
> SPOT Holder
The SPOT holder is compatible with standard liquid handlers manufactured by companies such as Beckman Coulter and Tecan Instruments.

CloneSaver Starter Kit

Includes: 2 CloneSaver Cards, 2 Uni-Core Punches (2 mm) with cutting mat and instructions.

Ordering Information - Clone Archiving
Catalogue Number Description Cards/Pack Sample Areas/Card Maximum Volume/Sample Area (µL) Maximum Total Volume/Card (µL)
WB120052 CloneSaver Starter Kit N/A N/A N/A N/A
WB120028 CloneSaver Card 5 96 well format 5 480
WB100024 CloneSaver Resealable Multi-Barrier Pouch 50 N/A N/A N/A
WB100034 SPOT Holder for Semi-Automated Spotting 1 N/A N/A N/A
 
 
Clone archiving
> EasyClone 384 Plate
EasyClone 384™ Plate
The Whatman EasyClone 384 replaces traditional freezer storage methods and offers first of its kind, single device for entire sample archiving and purification workflow, enabling faster DNA mining and discovery. 

The EasyClone 384 plate can be used by biotechnology, pharmaceutical, government and academic laboratories for the archiving, shipping and purifying of clones - all at room temperature.

Relying on proven Whatman FTA® technology, which allows for the collection, storage and purification of DNA from a variety of biological samples, EasyClone consists of a 384-well storage and extraction plate with a piercable foil bottom and FTA disks pre-cut into each well.  The design and format of the EasyClone 384 plate enables the genomics market to use FTA as a replacement for both ultra-low archiving and purification kits.

Ordering Information - EasyClone 384™ Plate
Catalogue Number Description Quantity/Case
WB120069 EasyClone 384 plates 50
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Multiwell accs.

UNIFILTER® Filtration Microplates

UNIFILTER® Filtration Microplates
> UNIFILTER Microplate
The patented Whatman UNIFILTER microplates with filter-bottom wells are convenient and ready to use. Available in 24, 96 and 384 well formats, UNIFILTER microplates offer a choice of filter media to meet exact application requirements. 

The unique drip director design of Whatman UNIFILTER microplates ensures precise collection of the filtrate to allow for further processing and analysis. UNIFILTER microplates are available in a range of well volumes from 100 µL to 10 mL.

Features and Benefits

  • No crosstalk. Patented integral filter design prevents well-to-well cross contamination
  • Economical to use. Wide range of well volume options ensures efficient use of materials
  • Better control. Choice of filter media allows control of the flow rates and retention characteristics
  • Versatile. A broad range of filtration media is available including glass fibre, polypropylene, cellulose nitrate, cellulose acetate, nylon and ion exchange cellulose
Typical Data - UNIFILTER
Filter Media Flow Rate* Protein Binding Hydrophilic Solvent Resistance Physical Strength Thermal Resistance °C General Comments
Cellulose Nitrate (CN) 4 High Yes Poor Brittle <125 Highly adsorptive membrane (CN) typically used for DNA/RNA/ protein hybridization, also for ELISA and RIA-based assays.
Cellulose Acetate (CA) 3 Low Yes Poor Moderate <120 Typically used for low protein binding applications, good wet strength. General purpose microbiological filter.
Polypropylene (PP) 2 Negligible No Very Good Good <80 Typically used for prefiltration. Sensitive to gamma sterilization. Very low extractables, chemically inert.
Polyvinylidene fluoride (PVDF) Hydrophilic** 4 Low Yes Good Good <135 Low protein binding, good chemical resistance.
Glass Microfiber (GF) 5 Moderate Yes Very Good Poor High Wide range available. Typically used as absorptive or adsorptive wicking media and prefilters. Excellent particle retention and resistance to clogging. Used for DNA binding.
 
 
 

96 Well 800 µL UNIFILTER microplate

UNIFILTER® filtration microplates
> 96 Well 800 µL UNIFILTER microplate
96 Well 800 µL UNIFILTER microplate
The 800µL UNIFILTER is the microplate most typically used in purifications, isolations and separation of biomolecules, particularly DNA. 

The microplate has a well volume of 800µL which is ideal for standard DNA plasmid miniprep chemistries. The choice of short or long drip directors is application specific. The UNIFILTER 800µL is constructed from rigid high grade polystyrene.

96 Well 800 µL UNIFILTER® Microplate
Catalogue Number Well Format Well Volume (µL) Plate Material Drip Director Filter Media Quantity/Case
7700-1801 96 800 Clear Polystyrene Short Whatman GF/C 25
7700-1804 96 800 Clear Polystyrene Short 25–30 µm melt blown polypropylene 25
7700-1806 96 800 Clear Polystyrene Short 0.45 µm hydrophilic PVDF 25
7700-1808 96 800 Clear Polystyrene Short 0.45 µm Cellulose Acetate 25
7700-1818 96 800 Clear Polystyrene Short 5–7 µm melt blown polypropylene 25
7700-2801 96 800 Clear Polystyrene Long Whatman GF/C 25
7700-2803 96 800 Clear Polystyrene Long Whatman GF/B 25
7700-2804 96 800 Clear Polystyrene Long 25–30 µm melt blown polypropylene 25
7700-2805 96 800 Clear Polystyrene Long 0.45 µm PP membrane 25
7700-2806 96 800 Clear Polystyrene Long 0.45 µm hydrophilic PVDF 25
7700-2808 96 800 Clear Polystyrene Long 0.45 µm Cellulose Acetate 25
7700-2809 96 800 Clear Polystyrene Long 0.45 µm Nylon Positive 25
7700-2810 96 800 Clear Polystyrene Long DNA binding plate 25
7700-2811 96 800 Clear Polystyrene Long Whatman GF/D 25
7700-2817 96 800 Clear Polystyrene Long 10–12 µm melt blown polypropylene 25
7720-2830 96 800 Clear Polystyrene Long Lysate clarification plate 25
7700-2828 96 800 Clear Polystyrene Long Whatman oleophobic PKP 10
7770-0062 96 800 Clear Polystyrene Long 25 µm melt blown polypropylene over 0.45 µm PP membrane 25
 
  • Multiwell accessories
 
Multiwell accessories
> BugStopper microplate capmat
BugStopper™ microplate capmat
Whatman BugStopper™ Capmats provide a simple and reliable method for venting cultures being grown in a 24 well microplate. This reusable sterile closure, which is produced using chemically resistant biosafe silicone rubber, incorporates hydrophobic microfilters which provide an ideal vent for each well.
More efficient than plastic lids, test comparisons confirm that BugStopper Capmats improve cell growth and significantly reduce evaporation. The silicone rubber portion of the capmat reseals after puncture, thus keeping cell cultures sterile during inoculation or aspiration.

Sterile Venting Closures for Microplate Cultures

  • More efficient than plastic lids. Perfect for extended growth of slow growing bacteria and fungi
  • Positive seal for every well. Significantly reduces evaporation rate and eliminates well-to-well cross-contamination
  • Autoclave and re-use. Cost-effective; repeated autoclave cycles do not affect gas exchange or retention capabilities
  • Rated 99.9% efficient for bacteria and viruses. Restricts microorganisms while allowing H2O and CO2 to pass through the membrane
  • Prevents aerosol formation. Suitable for growth of infectious microorganisms
Ordering Information
Catalogue Number Well Format Item Material Quantity/Case
7704-0014 24 BugStopper Venting Capmat for 10 mL Microplates Silicone Rubber 5
7701-5102 24 Growth Plate, 10 mL, round-bottom Polypropylene 25
 

  • membrane filters
 

membrane filters

Membrane filters
 membrane filters
Whatman brings to the laboratory user a range of membrane filters whose advanced technical specifications makes them today's preferred choice for a wide range of applications. The membrane filters offer accurately controlled pore size distribution and higher strength and flexibility which ensure reproducibility and consistency. The Whatman membrane filter range includes pore sizes (from 0.02 to 5µm) with a wide selection of membrane filters. Sterile and autoclave packs are available for specialized applications. Colored and gridded types are also available.

To access the range of Whatman Membrane Filters, please click on the links on the navigation box to the right.

 

Anodisc inorganic membranes

Anopore® inorganic membranes
> Anodisc inorganic membranes
The Anopore® inorganic membrane is ideal for a wide range of laboratory filtration applications. This unique material has a precise, non-deformable honeycomb pore structure with no lateral crossovers between individual pores.that filters at precisely the stated cutoff, allowing no larger sized particles to pass through the membrane. The Anopore inorganic membrane is composed of a high purity alumina matrix that is manufactured electrochemically. The membrane also exhibits low protein binding, has minimal autofluorescence, is non-toxic and supports cellular growth. 

The precise pore structure and narrow pore size distribution of the Anopore membrane ensure a high level of particle removal efficiency. Micro-organisms and particulate material are captured on the surface of the membrane for subsequent analysis by light or electron microscopy. When wet, the membrane is virtually transparent, which means that retained particles do not need to be transferred to another surface before microscopic examination.

> Anodisc pore structure

Anopore® inorganic membranes
> Anodisc pore structure

The membrane is hydrophilic and is compatible with most solvents and aqueous material. No monomers, plasticizers, adhesives, surfactants or wetting agents are used in the manufacturing process, which eliminates sample contamination and ensures low protein binding and minimal loss of sample.

The Anopore membrane is supplied in the form of Anodisc membrane filters. The membrane is peripherally bonded to an annular polypropylene ring (except the 13 mm diameter disc) for ease of handling and is suitable for both vacuum and pressure filtration.

Anopore is available in 3 nominal pore sizes: 0.02µm, 0.1µm and 0.2µm and in 3 disposable syringe filter housings: 13mm, 25mm and 47mm.

Features and Benefits

  • High pore density and narrow pore size distribution make it an extremely precise membrane
  • Wide solvent compatibility reduces the need to stock a variety of membranes in the laboratory
  • No additives used in the manufacturing process ensures minimal extractables and no sample contamination
  • Extremely low protein binding minimizes sample loss
  • Virtually transparent when wet making it ideal for microscopy studies

Applications

  • HPLC mobile phase filtration and degassing
  • Ultra cleaning of solvents
  • Gravimetric analysis
  • Liposome extrusion
  • Scanning electron microscopy studies
  • Bacterial analysis by epifluorescence light microscopy
  • Micrometer and nanometer filtration
  • Metal nanorods formation
Typical Data
  Anodisc 13 Anodisc 25 Anodisc 47
Average Membrane Thickness 60 µm 60 µm 60 µm
Membrane Diameter 13 mm 21 mm 43 mm
Membrane Type Anopore    
  aluminum oxide    
Support Ring Material None Polypropylene Polypropylene
Construction Process None Thermal weld Thermal weld
Protein Adsorption Low Low Low
Burst Strength 65–110 psi 65–110 psi 65–110 psi
Maximum Service Temperature 400°C 400°C 400°C
Porosity 25-50% 25-50% 25-50%
Autoclavable Yes No No
Refractive Index 1.6 1.6 1.6
Ordering Information - Diameter (mm)
Diameter (mm) Pore Size (µm) Membrane Catalogue Number Hydrophilic Protein Binding Solvent Resistance Quantity/Pack
13 Anodisc 13* 0.02 6809-7003 Yes Low Very Good 100
13 Anodisc 13* 0.1 6809-7013 Yes Low Very Good 100
13 Anodisc 13* 0.2 6809-7023 Yes Low Very Good 100
25 Anodisc 25 0.02 6809-6002 Yes Low Very Good 50
25 Anodisc 25 0.1 6809-6012 Yes Low Very Good 50
25 Anodisc 25 0.2 6809-6022 Yes Low Very Good 50
47 Anodisc 47 0.02 6809-5002 Yes Low Very Good 50
47 Anodisc 47 0.1 6809-5012 Yes Low Very Good 50
47 Anodisc 47 0.2 6809-5022 Yes Low Very Good 50
 
 

  • Application specific plates
     

 

Application specific plates
> ELISA UNIFILTER
ELISA UNIFILTER®
The Whatman ELISA plate allows researchers to use the binding characteristics of nitrocellulose in the standard SBS 8 x 12 multiwell format. It is also suitable for other types of ligand binding applications such as hybridoma supernatant screening. Solutions are easily vacuumed to waste using a vacuum manifold.
Chemiluminescent assays can be read in the plate. If the ELISA product is eluted then centrifugation is preferred. This microplate is compatible with automated systems.
Ordering Information - ELISA UNIFILTER®
Catalogue Number Well Format Well Volume Plate Material Filter Media Well Bottom Quantity/Case
7700-3307 96 350 µL White Polystyrene 0.45µm CN * Filter 50
7701-1350 96 300 µL Clear Polystyrene N/A Flat 50
7701-5200 96 2 mL Natural Polypropylene N/A Round 25
7705-0107 UniVac™ Vacuum to Collect Manifold, acrylic 1
7704-0001 Clear Polyester ThinCold Sealing Film Adhesive Backing 100
* CN = Cellulose nitrate
 

  • Protein precipitation UNIFILTER FF
 
Application specific plates
> Protein precipitation UNIFILTER FF
 

Protein precipitation UNIFILTER® FF

The Whatman Protein Precipitation UNIFILTER FF (Fast Flow)is optimized for removing acetonitrile-precipitated proteins from plasma or serum samples. Made with 2 mL, 96 well, rigid glass-filled polypropylene microplates, the Whatman Protein Precipitation UNIFILTER is both robust and chemically resistant.
 

The plates contain specially formulated dual membranes with two distinct layers. The top layer acts as a prefilter to remove coarse particulates. The bottom layer is oleophobic for retaining the well contents without dripping. This provides a final filter for removing fine particulate matter when a vacuum is applied. The plates are available in the following well bottom formats: Standard; Fast Flow; Round Bottom and "V" Bottom.

Features and Benefits
 
  • 96 samples purified at the same time
  • Purified samples available in less than 10 minutes
  • Precipitation and filtration in the same well
  • No laborious pipetting and/or centrifugation required and minimal liquid handling
  • Dual Whatman filter media
  • Ensures no fluid loss during incubation and fast flow during filtration
  • Over 98% protein removal
  • Sample volumes up to 150µL for plasma and 200µL for serum
  • SBS compatible with the exception of the "V" bottom plate
  • Robotics friendly
Ordering Information - Protein Precipitation UNIFILTER® FF
Catalogue Number Well Format Well Volume Plate Material Well Bottom Quantity/Case
7720-7235 96 2 mL Glass Polypropylene Standard 1
7720-7236 96 2 mL Glass Polypropylene Fast Flow 5
7701-5750 96 750 µL Natural Polypropylene Round Bottom 25
7701-5200 96 2 mL Natural Polypropylene Round Bottom 25
 
 
Application specific plates
> 384 Well DNA binding UNIFILTER
384 Well DNA binding UNIFILTER®
The 384 Well DNA Binding UNIFILTER plate effectively binds and purifies DNA molecules. It provides highly reproducible results with yields exceeding 2 µg/well, from bind-wash-elute processing with collection by filtration. Minimal liquid hang up allows for reduced elution volume, enabling DNA concentration as high as 150 ng/µL. Further ethanol precipitation is unnecessary. The DNA is ready to use.
 

Features and Benefits

  • High efficiency bind-wash-elute processing with collection by filtration
  • simplifies automation with no cross contamination
  • Highly reproducible results yielding DNA purity exceeding 2µg per well sufficient for sensitive downstream applications
  • Minimal liquid hang up allows for reduced elution volume with DNA concentration as high as 150ng/µL
Ordering Information - 384 Well DNA Binding UNIFILTER®
Catalogue Number Well Format Well Volume (µL) Plate Material Filter Media Quantity/Case
7700-2110 384 100 Clear Polystyrene DNA Binding 50
7701-1100 384 100 Clear Polystyrene N/A 50

Flexible capmats
 
Multiwell accessories
> Flexible capmats
Flexible capmats
Whatman flexible capmats individually seal the top of each well. Capmats may be used on either filter or collection microplates. 
 
Ordering Information
Capmats
Catalogue Number Well Format Capmat Material Microplate Compatibility Quantity/Case
7704-0004 96 Square Format EVA 2 mL microplates 100
7704-0005 96 Round Format EVA 750 µL and 800 µL microplates 100
7704-0006 48 Rectangular Format EVA 5 mL microplates 100
7704-0007 24 Square Format Santoprene 10 mL microplates 100
7704-0015 384 Square Format Santoprene 400 µL microplate 100
Pierceable Capmats
7704-0104 96 Square Format Silicone 2 mL microplates 50
7704-0105 96 Round Format Silicone 300 µL, 750 µL and 800 µL microplates 50
7704-0115 384 Square Format Silicone 100 µL and 400 µL microplates 50
Venting Capmats (autoclavable)
7704-0014 24 BugStopper Venting 10 mL microplates 5
 
 		  
 
 

 Chromatography cellulose media

Chromatography cellulose media
> Chromatography cellulose media
Whatman offers an extensive product line of chromatography cellulose media for applications ranging from the separation of biopolymers such as proteins, peptide and hormones to the purification of monoclonal antibodies, vaccines, synthetic pharmaceuticals and agrochemicals. 

Technological innovations in product development and manufacturing processes and a dedication to excellence are combined to ensure batch-to batch reproducibility of these chromatography cellulose media.
 
 

Bulk silica media for column chromatography

Bulk silica media for column chromatography
> Bulk silica media for column chromatography
The Whatman bulk silica media is used for low pressure columns. Same base silica and chemistries as those used for Whatman TLC plates. Particularly useful for scaling up separations optimized by TLC. In addition, Whatman offers an all-purpose media for general column chromatography. Applicable for preparative low pressure column separations scaled up from Partisil analytical HPLC columns.
 

Features and Benefits

  • High resolution
  • Good flow characteristics
  • High surface area
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for Low Pressure Column Chromatography
4771-010 LPS-2 low pressure silica gel 37–53 Adsorption 450 m2/g; may be dry packed 1 kg
4776-001 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 100 g
4776-005 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 500 g

 
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for General Column Chromatography
4132-100 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 100 g
4132-301 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 1 kg
4790-010 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 1 kg
4790-050 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 5 kg
4790-250 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 25 kg
4791-005 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 500 g
4791-010 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 1 kg
4791-050 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 5 kg
4791-250 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 25 kg

NEW Purasil 60Å Silica Gel

Silica Gel Media for Flash Chromatography 
Whatman Purasil high purity silica gel provides an excellent separation medium for flash chromatography purification of target molecules. Narrow particle size distribution and minimal fines enable fast separations with no loss of chromatographic performance.

Features and Benefits

  • High resolution
  • Excellent flow rates
  • Direct scalability
Typical Data
Iron Content <0.02%
Chloride Content <0.10%
Loss on Drying <7%
pH (10% suspension) 7 ± 0.5
Pore Volume 60–80 mL/g
Surface Area 500–600 m2/g
Ordering Information
Catalogue Number Product Dimensions
4745-010 230–400 mesh (38–63 µm) 1 kg
4745-250 230–400 mesh (38–63 µm) 25 kg
4746-010 70–230 mesh (63–210 µm) 1 kg
4746-250 70–230 mesh (63–210 µm) 25 kg
 
 
 

Collection plates

Collection plates
> Collection plates
Whatman microplates for collection and analysis are available in 24, 48, 96 and 384 well formats, all unique to Whatman. These microplates are manufactured from polystyrene, polypropylene and Multi-Chem™ materials to accommodate a wide range of sampling and storage applications. 

To access the range of Whatman Collection Plates, please click on the links on the navigation box to the right.
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