Friday, 07 November 2014 00:00

ExiSpin™


ExiSpin™ (*NEW*)

Essential Reproducibility, Enhanced Reliability, Excellent Results
instrumentfigure1

Description
ExiSpin has the functions of a vortexer as well as a microcentrifuge and will significantly save you time in the lab.ExiSpin comes complete with rotors for 4 x 8-strip tubes and 12 x 1.5/2.0 ml tubes. Use it for Minipreps, to resuspend oligos, set up you PCR reactions, or any task that requires vortexing in micro test tube or 0.2 ml PCR tubes.

Once you try the ExiSpin, you will find it an essential instrument for you laboratory. Contact your local sales representative for a demo today!

 

Features and Benefits

Full speed vortex and centrifuge: No need to vortex by hand and load a centrifuge to spin down
Programmable: Flexible programming ensures reproducible mixing
Economical: For less than the cost of a Vortexer, you get a Centrifuge too!



• Fully Automated Spin-Mix-Spin Technology
The time and strength of centrifugation/mixing is fully programmable. The numbers of spin-mix-spin cycles can be set (1-999). After this input, a single button automatically starts the process as follows:

1. 1st spin: Spin down samples to a bottom of tube
2. Vortexing of samples
3. 2nd spin: Spin down mixed samples to a bottom of tube

 

Convenience and High Reproducibility
It provides reproducible results using protocols optimized to samples by preventing the possibilities of experimental errors. Four 8-strips tubes can be handled simultaneously, which enable 32 PCR/QPCR mixture tubes ready to run at once. Twelve 1.5 ml tubes can be loaded for simultaneous cell lysis and subsequent centrifuge-down. The programmed speed and time quarantine the reproducibility of each application.

 

Economical and Efficient
ExiSpin has both a centrifuge and vortex function. You can save not only your time but also the budget by purchasing one instead of two separate instruments. 12 x 1.5 ml rotor and 4 x 8-strip rotor (Figure. 1) are easily changed and both rotors are included in the basic system.


instrumentfigure12

Figure 1.Presented rotors for 8-strip PCR tube [left] and 1.5 ml microtube [right]

 

Price: 1 355 USD

Published in Lab Instruments
Friday, 07 November 2014 00:00

Exicycler 96

Exicycler™ 96 Quantitative Real-Time PCR System

superior optics for true 5-color performance
Exicycler96

Description
Exicycler is a flexible, 5-color system with advanced features such as a thermal gradient function as well as light tunnel technology which eliminates the need for a reference dye. 

Exicycler 96 is different from most Real Time systems in that we’ve addressed a significant problem that affects many systems – edge effects and the need for reference dyes. Most systems illuminate the wells from above which results in up to a 30% difference in light intensity from the center of the plate to the edges. Gentaur’s Exicycler 96 uses our exclusive light tunnel technology to deliver the same number of photons to each well, eliminating the need to use reference dyes to adjust for intensity differences! 

overview2


Exicycler 96 also offers several other features such as a thermal gradient for optimization. This is a critical feature to have for optimization of real time PCR and absolutely essential for multiplex optimization.
Exicycler 96 has all the analysis modules you would expect in a Real-Time system including; Absolute Quantification, Relative Quantification, +/- Assays, SNP Genotyping, Melt Curve Analysis, etc. 
By using a high-sensitivity 2D 16-bit CCD sensor and high-quality optics, Exicycler 96 is able to acquire clean and sensitive signals from all the wells simultaneously. All fluorescent data is saved regardless of how the experiment was set up, which means that if a well was accidentally left out, the data can be recovered, or rather, rediscovered! 

overview3

overview4 


Features and Benefits

Light Tunnel Technology: Provides homogenous illumination across the plate
True 5-color Multiplexing: No need to run reference dyes or plates
Bright, Short Arc lamp: Longer life and even spectrum compared to Halogen bulbs
Open system: Use any Reagents or consumables*
Gradient function: Up to 20°C across the block. Essential for optimization
Fluorescence data obtained from 
96 wells simultaneously:
No time-lag between wells

 

Price: 43 000 USD

Published in Lab Instruments
Friday, 07 November 2014 00:00

EXIPROGEN

ExiProgen is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run. 
DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein. 
The crude protein extract is further purified, using His-Tag engineered into the proteins. 
The net result is up to 100 ug of >90% pure Protein in about 6 hours. 
ExiProgen can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.

 

Features and Benefits

Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples
ExiProgen has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols

 

figure2Cooling Block 
ExiProgen has a built-in cooling block where the elution tube rack sits. 
Sample integrity is ensured by keeping the samples below 10°C.
This allows for overnight runs and provides you with confidence in your results.






figure3Magnetic block & Heating block
To increase extraction efficiency, the ExiProgen has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.







figure4Contamination Shield
ExiProgen comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.







figure4Easy to use LCD Touch screen 
The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.








figure3UV lamp
ExiProgen has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.







Experimental Procedure

figure6

Principle of protein synthesis and purification

ExiProgen Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure. 

figure7

 

Nucleic acid extraction principle

ExiProgen DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA. 

figure8

 

 

Price: 20 250 USD

Published in Lab Instruments
Thursday, 30 October 2014 00:00

IM9702 Spoligotyping Membrane

very simple             -             inexpensive             -             effective

 for Tuberculosis/ Mycobacterium research

Spoligotyping is a useful PCR-Based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.).

The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies.

Price: 1395 USD

Published in Spoligotyping

A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research.

Spoligotyping is a useful PCR-Based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.).

The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies.

Price: 1466 USD

Published in Spoligotyping
Friday, 24 October 2014 00:00

Control of animal disease - Avian

   - Canine

   - Feline

   - Bovine

   - Porcine

   - Avian

 

 

 

 

Published in Promotions

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