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CloneSaver™ Card
FTA® Technology in 96 Well Format for High Throughput Applications
Designed for the collection, long-term storage and purification of plasmid and BAC DNA from bacterial clones in a 96 well format.
Prepare BAC and Plasmid DNA with Amazing Ease
- Apply 5 µL bacterial culture, re-suspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing
- Bacteriophages are inactivated
- DNA is easily accessible for downstream applications
- Store up to 96 samples on each card
Store Sample DNA for Years at Room Temperature
Plasmid DNA stored on CloneSaver Cards is stable at room temperature for at least 4 years...and counting.
DNA is Easily Accessible for Downstream Applications
Transformation
Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods.
PCR
Immobilized plasmid DNA on a CloneSaver Card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies.
Sequencing
Plasmid DNA eluted from a CloneSaver punch can be amplified by rolling circle amplification, such as Amersham Bioscience's TempliPhi™ and then sequenced without the need for culture re-growth and plasmid purification.
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| > CloneSaver Card with Multi-Barrier Pouches |
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CloneSaver™ Resealable Multi-Barrier Pouches
Used for transporting or storing the CloneSaver Card. The pouch is constructed with seven laminated layers that protects the card from exposure to gas or liquid contamination. There is a zip-lock resealable closure for easy access to the CloneSaver Card. The tamper-evident seal maintains sample security and the outer paper surface can be used for labelling or writing.
SPOT CloneSaver/BloodSaver Holder
SPOT is a rigid frame that allows automated spotting to standard CloneSaver and BloodSaver cards. It keeps the card flat for uniform and precise spotting of your biological samples. A 96 well card is easily inserted into the SBS-compatible frame, which can then be placed onto a liquid-handling deck just like a multiwell plate.
The SPOT holder is compatible with standard liquid handlers manufactured by companies such as Beckman Coulter and Tecan Instruments.
CloneSaver Starter Kit
Includes: 2 CloneSaver Cards, 2 Uni-Core Punches (2 mm) with cutting mat and instructions.
| Ordering Information - Clone Archiving |
| Catalogue Number |
Description |
Cards/Pack |
Sample Areas/Card |
Maximum Volume/Sample Area (µL) |
Maximum Total Volume/Card (µL) |
| WB120052 |
CloneSaver Starter Kit |
N/A |
N/A |
N/A |
N/A |
| WB120028 |
CloneSaver Card |
5 |
96 well format |
5 |
480 |
| WB100024 |
CloneSaver Resealable Multi-Barrier Pouch |
50 |
N/A |
N/A |
N/A |
| WB100034 |
SPOT Holder for Semi-Automated Spotting |
1 |
N/A |
N/A |
N/A |
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