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price : 5 slide for 600 $
CYN1 Human, Normal Endometrium | ||||
No. | Age | Sex | Phase | Associated lesion |
1 | 35 | F | proliferative | squamous cell carcinoma of cervix |
2 | 39 | F | proliferative | leiomyoma |
3 | 40 | F | proliferative | squamous cell carcinoma in situ of cervix |
4 | 40 | F | secretory | leiomyoma |
5 | 33 | F | secretory | leiomyoma |
6 | 45 | F | secretory | leiomyoma |
7 | 40 | F | secretory | leiomyoma |
8 | 39 | F | secretory | leiomyoma |
9 | 38 | F | proliferative | endometriosis, ovary |
10 | 42 | F | secretory | leiomyoma |
11 | 44 | F | secretory | leiomyoma |
12 | 45 | F | secretory | leiomyoma |
13 | 37 | F | secretory | leiomyoma |
14 | 38 | F | proliferative | leiomyoma |
15 | 38 | F | proliferative | leiomyoma |
16 | 42 | F | secretory | squamous cell carcinoma of cervix |
17 | 38 | F | proliferative | leiomyoma |
18 | 37 | F | proliferative | leiomyoma |
19 | 41 | F | proliferative | leiomyoma |
20 | 34 | F | secretory | squamous cell carcinoma in situ of cervix |
21 | 43 | F | secretory | leiomyoma |
22 | 40 | F | proliferative | leiomyoma |
23 | 42 | F | secretory | leiomyoma |
24 | 41 | F | proliferative | moderate dysplasia of cervix |
25 | 45 | F | proliferative | leiomyoma |
26 | 30 | F | secretory | squamous cell carcinoma of cervix |
27 | 39 | F | proliferative | leiomyoma |
28 | 43 | F | secretory | leiomyoma |
29 | 36 | F | proliferative | leiomyoma |
30 | 44 | F | secretory | leiomyoma |
31 | 42 | F | proliferative | leiomyoma |
32 | 30 | F | secretory | leiomyoma |
33 | 41 | F | secretory | leiomyoma |
34 | 33 | F | secretory | leiomyoma |
35 | 37 | F | secretory | normal |
36 | 29 | F | secretory | squamous cell carcinoma in situ of cervix |
37 | 42 | F | secretory | leiomyoma |
38 | 37 | F | proliferative | squamous cell carcinoma in situ of cervix |
39 | 44 | F | proliferative | leiomyoma |
40 | 35 | F | proliferative | adenomyoma |
41 | 32 | F | secretory | leiomyoma |
42 | 36 | F | secretory | cellular leiomyoma |
43 | 37 | F | secretory | leiomyoma |
44 | 35 | F | secretory | leiomyoma |
45 | 37 | F | secretory | squamous cell carcinoma in situ of cervix |
46 | 42 | F | secretory | leiomyoma |
47 | 44 | F | proliferative | leiomyoma |
48 | 40 | F | proliferative | cellular leiomyoma |
49 | 35 | F | proliferative | squamous cell carcinoma of cervix |
50 | 36 | F | proliferative | squamous cell carcinoma of cervix |
51 | 35 | F | secretory | squamous cell carcinoma of cervix |
52 | 43 | F | secretory | squamous cell carcinoma of cervix |
53 | 42 | F | proliferative | leiomyoma |
54 | 35 | F | proliferative | squamous cell carcinoma of cervix |
55 | 43 | F | secretory | leiomyoma |
56 | 42 | F | secretory | squamous cell carcinoma of cervix |
57 | 33 | F | proliferative | leiomyoma |
58 | 35 | F | proliferative | adenomyosis |
59 | 45 | F | secretory | leiomyoma |
60 | carbon |
General Information of the Tissue Array Slide :
Applications
- Immunohistochemistry
- In situ hybridization, RNA or DNA
- Fluorescent in situ hybridization (FISH)
- In situ PCR, RNA or DNA
- TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)
Storage and stability
- Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
- Tissue array slides are shipped in room temperature.
- If the slides are stored in 4 degree, they are good up to one year.
How processed
- Tissues were initially fixed with formalin except for some of the animal tissues.
- Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
- Cleared by xylene, three changes for 1 hour each.
- Infiltrated with 60°C paraffin, three changes for 1 hour each
- Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)
Before use
- Dry slides for 1 hour in a oven at 60 degree.
- Dewax slides in xylene for 4 minutes x 5 times
- Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
- Immerse slides in tap water for 5 minutes
Slide orientation
- In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.
Shaded area |
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31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 |
51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |